Recombinant Ovine Anti-CarLA Antibody (PAB-1)
CAT#: FAMAB-0264-CN
This is a sheep monoclonal antibody against CarLA, a carbohydrate larval antigen which is present on the exposed surface of all strongylid nematode infective L3 larvae tested. The antibody PAB-1 recognized CarLA at a non-surface epitope.
Published Data
WB
Figure 1 Western blots of nine selected ovine scFvs for recognition of antigens in T. colubriformis L3 larval extracts.
Soluble nematode L3 larval extract from T. colubriformis was separated on 11% SDS-PAGE and probed with the specified scFvs or PAB-1 (antiCarLA mAb). The indicated scFvs were incubated with the blots and bound scFv detected using an anti-E-tag/HRP conjugated mAb. scFv members recognizing the three epitope groups, E1, E2 and E3, are indicated at the top.
Maass, D. R., Harrison, G. B., Grant, W. N., Hein, W. R., & Shoemaker, C. B. (2009). Intraspecific epitopic variation in a carbohydrate antigen exposed on the surface of Trichostrongylus colubriformis infective L3 larvae. PLoS pathogens, 5(9).
ELISA
Figure 2 Biopanning results in the identification of phage clones that bind to mAb PAB1.
Immobilised mAb PAB1 recognised a selection of purified phage clones diluted 1:20, negative control: VCSM13.
Umair, S., Deng, Q., Roberts, J. M., Shaw, R. J., Sutherland, I. A., & Pernthaner, A. (2016). Identification of peptide mimics of a glycan epitope on the surface of parasitic nematode larvae. PloS one, 11(8).
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Specifications
- Immunogen
- Carbohydrate surface antigen of T. colubriformis L3 larvae, CarLA
- Host Species
- Ovine
- Derivation
- Phage display library
- Type
- Ovine IgG
- Specificity
- Trichostrongylus colubriformis CarLA
- Species Reactivity
- Trichostrongylus colubriformis
- Clone
- PAB-1
- Applications
- WB, ELISA
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
- Alternative Names
- CarLA
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0265-CN | Recombinant Ovine Anti-CarLA Antibody (Tc.C1) | WB, ELISA, IF, IB | Ovine IgG |
| FAMAB-0266-CN | Recombinant Ovine Anti-CarLA Antibody (Tc.E9) | WB | Ovine IgG |
| FAMAB-0267-CN | Recombinant Ovine Anti-CarLA Antibody (Tc.C3) | WB, ELISA, IF, IB | Ovine IgG |
| VS-0423-XY4 | Sheep Anti-CarLA Recombinant Antibody (clone Tc.C10) | ELISA, WB, IF | Sheep IgG |
| VS-0423-XY5 | Sheep Anti-CarLA Recombinant Antibody (clone Tc.B2) | ELISA, WB, IF | Sheep IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0264-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (PAB-1) | WB, ELISA, IF, IB | Ovine scFv |
| FAMAB-0265-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (Tc.C1) | WB, ELISA, IF, IB | Ovine scFv |
| FAMAB-0266-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (Tc.E9) | WB, ELISA, IF, IB | Ovine scFv |
| FAMAB-0267-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (Tc.C3) | WB, ELISA, IF, IB | Ovine scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0264-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (PAB-1) | WB, ELISA, IF, IB | Ovine Fab |
| FAMAB-0265-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (Tc.C1) | WB, ELISA, IF, IB | Ovine Fab |
| FAMAB-0266-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (Tc.E9) | WB, ELISA, IF, IB | Ovine Fab |
| FAMAB-0267-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (Tc.C3) | WB, ELISA, IF, IB | Ovine Fab |
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