Recombinant Ovine Anti-CarLA Antibody (Tc.C1)
CAT#: FAMAB-0265-CN
This is a sheep monoclonal antibody against CarLA, a carbohydrate larval antigen which is present on the exposed surface of all strongylid nematode infective L3 larvae tested. The anti-CarLA antibody Tc.C1 is specific to the T. colubriformis species of nematode.
Published Data
IF
Figure 1 Immunofluorescence of living T. colubriformis L3 larvae probed with selected anti-CarLA scFvs individually.
Live T. colubriformis exsheathed L3 larvae were probed with Tc.C1 scFv alone, bound scFv was stained with anti-E-tag/FITC conjugated mAb and detected by fluorescence microscopy.
Maass, D. R., Harrison, G. B., Grant, W. N., Hein, W. R., & Shoemaker, C. B. (2009). Intraspecific epitopic variation in a carbohydrate antigen exposed on the surface of Trichostrongylus colubriformis infective L3 larvae. PLoS pathogens, 5(9), e1000597.
WB
Figure 2 Species specificity of anti-CarLA scFvs representing the E1 epitope classe.
Western blots were performed containing hot water extracts of L3 stage larvae prepared from six different species of nematode. Species used were Trichostrongylus colubriformis (T. co), Haemonchus contortus (H. co), Cooperia curticei (C. cu), Caenorhabditis elegans (C. el), Nematodirus spatiger (N. sp) and Teladorsagia circumcincta (T. ci). The indicated scFvs were incubated with the blots and bound scFv was detected using an anti-E-tag/HRP conjugated mAb. Markers (M) indicate the molecular weight mobility shown on left.
Maass, D. R., Harrison, G. B., Grant, W. N., Hein, W. R., & Shoemaker, C. B. (2009). Intraspecific epitopic variation in a carbohydrate antigen exposed on the surface of Trichostrongylus colubriformis infective L3 larvae. PLoS pathogens, 5(9), e1000597.
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Specifications
- Immunogen
- Carbohydrate surface antigen of T. colubriformis L3 larvae, CarLA
- Host Species
- Ovine
- Derivation
- Phage display library
- Type
- Ovine IgG
- Specificity
- Trichostrongylus colubriformis CarLA
- Species Reactivity
- Trichostrongylus colubriformis
- Clone
- Tc.C1
- Applications
- WB, ELISA, IF, IB
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Immunoblot, Western Blot and Immunofluorescence.
Target
- Alternative Names
- CarLA
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone Tc.C1"
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0264-CN | Recombinant Ovine Anti-CarLA Antibody (PAB-1) | WB, ELISA | Ovine IgG |
| FAMAB-0266-CN | Recombinant Ovine Anti-CarLA Antibody (Tc.E9) | WB | Ovine IgG |
| FAMAB-0267-CN | Recombinant Ovine Anti-CarLA Antibody (Tc.C3) | WB, ELISA, IF, IB | Ovine IgG |
| VS-0423-XY4 | Sheep Anti-CarLA Recombinant Antibody (clone Tc.C10) | ELISA, WB, IF | Sheep IgG |
| VS-0423-XY5 | Sheep Anti-CarLA Recombinant Antibody (clone Tc.B2) | ELISA, WB, IF | Sheep IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0264-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (PAB-1) | WB, ELISA, IF, IB | Ovine scFv |
| FAMAB-0265-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (Tc.C1) | WB, ELISA, IF, IB | Ovine scFv |
| FAMAB-0266-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (Tc.E9) | WB, ELISA, IF, IB | Ovine scFv |
| FAMAB-0267-CN-S(P) | Recombinant Ovine Anti-CarLA Antibody scFv Fragment (Tc.C3) | WB, ELISA, IF, IB | Ovine scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0264-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (PAB-1) | WB, ELISA, IF, IB | Ovine Fab |
| FAMAB-0265-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (Tc.C1) | WB, ELISA, IF, IB | Ovine Fab |
| FAMAB-0266-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (Tc.E9) | WB, ELISA, IF, IB | Ovine Fab |
| FAMAB-0267-CN-F(E) | Recombinant Ovine Anti-CarLA Antibody Fab Fragment (Tc.C3) | WB, ELISA, IF, IB | Ovine Fab |
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