Mouse Anti-Cationic 2-aminobenzimidazolium hapten 4 Recombinant Antibody (clone 34E4) (CAT#: HPAB-S0010-YC)
Antibody 34E4 was generated against the cationic 2-aminobenzimidazolium hapten 4 and catalyzes this transformation with high rates and multiple turnovers 34E4 efficiently promotes the conversion of benzisoxazoles to salicylonitriles.
Specific Inquiry
Figure 1 Brønsted plot for the aqueous reactions of benzisoxazoles with acetate (□) or 34E4 (■).
Benzisoxazoles containing a nitro substituent at the 6- or 7-position deviate negatively from the correlation in 34E4 shown in Figure and appear to lie along a line that is roughly parallel to that through the data for the other substrates.
Hu, Y., Houk, K. N., Kikuchi, K., Hotta, K., & Hilvert, D. (2004). Nonspecific medium effects versus specific group positioning in the antibody and albumin catalysis of the base-promoted ring-opening reactions of benzisoxazoles. Journal of the American Chemical Society, 126(26), 8197-8205.
Figure 2 Free energy (∆G) diagrams for reactions of acetate with 4-nitrobenzisoxazole in gas phase, acetonitrile, water, and antibody 34E4.
Numbers in bold face are experimental values and those in italics are the averaged calculated values based on PCM and CPCM models.
Hu, Y., Houk, K. N., Kikuchi, K., Hotta, K., & Hilvert, D. (2004). Nonspecific medium effects versus specific group positioning in the antibody and albumin catalysis of the base-promoted ring-opening reactions of benzisoxazoles. Journal of the American Chemical Society, 126(26), 8197-8205.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- 34E4
- Applications
- Catalyze
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cationic 2-aminobenzimidazolium hapten 4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0010-YC-F(E) | Mouse Anti-Cationic 2-aminobenzimidazolium hapten 4 Recombinant Antibody (clone 34E4); Fab Fragment | Catalyze | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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