Mouse anti-CD274 antibody (clone 9B5F4) displayed high specificity against the CD274 protein of Human.
Figure 1 Mouse Anti-CD274 Antibody (ZG-0238U) in WB
Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate All lanes: PD-L1 antibody at 1: 2500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 55 kDa
Figure 2 Mouse Anti-CD274 Antibody (ZG-0238U) in IHC
IHC image of ZG-0238U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Mouse Anti-CD274 Antibody (ZG-0238U) in IF
Immunofluorescence staining of A549 cells with ZG-0238U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
Figure 4 Mouse Anti-CD274 Antibody (ZG-0238U) in FC
Overlay histogram showing 293 cells stained with ZG-0238U (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0190CL | Anti-Human CD274 Recombinant Antibody (PD1-AB1) | FC, ELISA, FuncS | |
TAB-0997CL-S(P) | Anti-Human CD274 Recombinant Antibody scFv Fragment (B60-55) | ELISA | Human antibody |
TAB-1002CL-S(P) | Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL-S(P)); scFv Fragment | ELISA, FC | Mouse scFv |
TAB-1003CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-1003CL-S(P)) | FC, ELISA | Human scFv |
TAB-417CQ | Anti-Human CD274 Recombinant Antibody (Durvalumab) | ELISA, IHC, FC, IP, IF, Inhib | IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0262CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Mouse IgG |
TAB-0262CL-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0262CL-S(P)) | ELISA, WB, SPR, FuncS | Mouse scFv |
TAB-1009CL-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1009CL-F(E)) | WB, IHC | Mouse Fab |
TAB-1010CL-F(E) | Anti-Human CD274 Recombinant Antibody Fab Fragment | FC | |
TAB-1011CL-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1011CL-F(E)) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0263CL | Human Anti-CD274 Recombinant Antibody (TAB-0263CL) | ELISA, WB, SPR, FuncS | Humanized IgG |
TAB-0264CL | Human Anti-CD274 Recombinant Antibody (TAB-0264CL) | ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib | Humanized IgG |
TAB-0265CL | Human Anti-CD274 Recombinant Antibody (TAB-0265CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
TAB-0266CL | Human Anti-CD274 Recombinant Antibody (TAB-0266CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
TAB-0267CL | Human Anti-CD274 Recombinant Antibody (TAB-0267CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0995CL | Human Anti-CD274 Recombinant Antibody (TAB-0995CL) | ELISA, FC | Chimeric (Mouse/Human) IgG1, κ |
TAB-0995CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P)) | ELISA, FC | Human scFv |
TAB-0995CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-324CQ | Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6) | IHC-P, FC, Neut, ICC | Mouse IgG2b, κ |
NEUT-325CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-206) | Neut | Human IgG1, κ |
NEUT-326CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-207) | ELISA, Neut | Human IgG1, κ |
NEUT-327CQ | Mouse Anti-CD274 Recombinant Antibody (clone MIH1) | FC, FuncS, Neut, IHC, IF | Mouse IgG1, κ |
NEUT-330CQ | Rat Anti-CD274 Recombinant Antibody (clone MIH5) | FC, FuncS, Neut, IHC-Fr, WB | Rat IgG2a, λ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-328CQ | Rat Anti-CD274 Recombinant Antibody (VMC171) | Block, FC | Rat IgG2 |
NEUT-329CQ | Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2) | FC, IF, Block | Rat IgG2b, κ |
NEUT-331CQ | Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1) | ELISA, Block | Mouse IgG1, λ |
NEUT-332CQ | Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009) | Block | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0548 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB) | ELISA | Rabbit IgG |
MOR-4049 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS) | ELISA | Rabbit IgG |
MOR-4050 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS) | ICC, IF, FC | Rabbit IgG |
MOR-4051 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS) | FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0193-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E)) | ELISA | Humanized Fab |
HPAB-0194-YC-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E)) | ELISA | Mouse Fab |
HPAB-0195-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E)) | SPR | Humanized Fab |
HPAB-0196-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E)) | FC | Human Fab |
HPAB-0197-YC-F(E) | Rabbit Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0197-YC-F(E)) | ELISA, FC | Rabbit Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0408-YC-VHH | Recombinant Humanized Anti-CD274 Single Domain Antibody (PDL1-56dAb) | ELISA, FC, BL | Human VHH |
NBD-0065-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (CBP112D) | ELISA | Camelid VHH |
NBD-0066-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (KN035) | ELISA, Inihb | Camelid VHH |
NBD-0067-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (Nb1) | ELISA, FC | Camelid VHH |
NBD-0068-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (Nb2) | ELISA, FC | Camelid VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0561LY | Human Anti-CD274 Recombinant Antibody (HPAB-0561LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
HPAB-0562LY | Human Anti-CD274 Recombinant Antibody (HPAB-0562LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
HPAB-0563LY | Human Anti-CD274 Recombinant Antibody (HPAB-0563LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
HPAB-0564LY | Human Anti-CD274 Recombinant Antibody (HPAB-0564LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
HPAB-0565LY | Human Anti-CD274 Recombinant Antibody (HPAB-0565LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-417CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody (Durvalumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
AFC-TAB-418CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody (Atezolizumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, BL | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0646-WJ-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0646-WJ-S(P)) | ELISA, Block | Human scFv |
HPAB-1666-FY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-1666-FY-F(E)) | FC | Human Fab |
HPAB-1667-FY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-1667-FY-F(E)) | FC | Human Fab |
HPAB-1668-FY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-1668-FY-F(E)) | FC | Human Fab |
FAMAB-0129WJ-S(P) | Human Anti-CD274 Recombinant Antibody (clone BMS-936559); scFv Fragment | ELISA, WB | Human scFv |
There are currently no Customer reviews or questions for ZG-0238U. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.