AbPlus™ Anti-CD3E Magnetic Beads (VS-0724-YC1433)
CAT#: VS-0724-YC1433
The AbPlus Anti-CD3E Magnetic Beads (VS-0724-YC1433) is an innovative affinity resin which is bound with anti-CD3E specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of CD3E protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-CD3E specific antibody
- Target
- CD3E
- Immunogen
- Recombinant human CD3e/CD3 epsilon, aa 1-126.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- CD3E (CD3e Molecule) is a Protein Coding gene. Diseases associated with CD3E include Immunodeficiency 18 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are G-protein signaling N-RAS regulation pathway and Toll-like receptor signaling pathway. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and signal transducer activity.
- Alternative Names
- CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3;
- Gene ID
- 916
- UniProt ID
- P07766
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Datasheet
MSDS
COA
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|---|---|---|---|
| TAB-H28 | Anti-Human CD3E Recombinant Antibody (Ertumaxomab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG2a / G2b |
| CAT | Product Name | Application | Type |
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| TAB-714 | Anti-CD3E Recombinant Antibody (Visilizumab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG2 |
| TAB-159 | Anti-Human CD3E Recombinant Antibody (TAB-159) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-245 | Anti-Human CD3 epsilon Recombinant Antibody (TAB-245) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG1 - kappa |
| TAB-124CL | Human Anti-CD3E Recombinant Antibody (TAB-124CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
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| TAB-019 | Anti-Human CD3 Recombinant Antibody (TAB-019) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG2a - kappa |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G010E | Anti-CD3E immunotoxin UCHT1 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G010D | Anti-CD3E immunotoxin UCHT1 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-L049E | Anti-CD3E immunotoxin (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L049D | Anti-CD3E immunotoxin (IgG)-DT | Cytotoxicity assay, Functional assay | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-600NL | Recombinant Anti-mouse CD3e Antibody. | IP, IHC-Fr, FuncS, ICC, FACS | |
| MRO-1744-CN | Rabbit Anti-CD3E Polyclonal Antibody (MRO-1744-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FN-098CQ | Mouse Anti-CD3E Recombinant Antibody (clone HIT3a) | FuncS | Mouse IgG2a, κ |
| HPAB-A1283-YJ | Hamster Anti-CD3E Recombinant Antibody (clone 145.2c11) | ELISA, WB, IP, FC, FuncS, Inhib | Hamster IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-643 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
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| FAMAB-0047CQ-S(P) | Mouse Anti-CD3E Recombinant Antibody (clone CLB-T3/4.1); scFv Fragment | ELISA, IF, IP | Mouse scFv |
| HPAB-0263-YJ-S(P) | Rabbit Anti-CD3E Recombinant Antibody (clone 645); scFv Fragment | ELISA, FC, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-032 | Recombinant Grey hamster Anti-CD3E Antibody (2C11) | WB, ELISA, FuncS, IP | IgG |
| PABX-033 | Recombinant Grey hamster Anti-CD3E Antibody (17A2) | WB, ELISA, FuncS | IgG |
| PABX-032-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (2C11) | WB, ELISA, FuncS | Fab |
| PABX-033-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (17A2) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0558 | Hi-Affi™ Rabbit Anti-CD3E Recombinant Antibody (clone DS558AB) | IHC-P | Rabbit IgG |
| MOR-0058-FY | Rabbit Anti-CD3E Recombinant Antibody (clone AFY0029) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0049-YC-F(E) | Hamster Anti-CD3E Recombinant Antibody Fab Fragment (FAMAB-0049-YC-F(E)) | FC, FuncS | Hamster Fab |
| FAMAB-0052-YC-F(E) | Hamster Anti-CD3E Recombinant Antibody (clone 500A2); Fab Fragment | ELISA | Hamster Fab |
| FAMAB-0047CQ-F(E) | Mouse Anti-CD3E Recombinant Antibody (clone CLB-T3/4.1); Fab Fragment | ELISA, IF, IP | Mouse Fab |
| HPAB-0263-YJ-F(E) | Rabbit Anti-CD3E Recombinant Antibody (clone 645); Fab Fragment | ELISA, FC, FuncS | Rabbit Fab |
| HPAB-0347-YJ-F(E) | Rat Anti-CD3E Recombinant Antibody (clone 1Ab); Fab Fragment | FC, WB, Inhib | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| AFC-TAB-245 | Afuco™ Anti-CD3E ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-245) | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-H52 | Afuco™ Anti-CD3E ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H52) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-714 | Afuco™ Anti-CD3E ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-714) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-019 | Afuco™ Anti-CD3E ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-019) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY17 | Mouse Anti-CD3E Agonistic Antibody (VS-0724-XY17) | FC, IP, ICC, Agonistic assays | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY94 | Mouse Anti-NHP CD3E Recombinant Antibody (clone FN-18) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY110 | Human Anti-CD3E (clone NI-0401) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0525-XY1171 | Anti-CD3E Immunohistochemistry Kit | IHC | |
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