Anti-CD3E immunotoxin UCHT1 (scFv)-DT

Name: Anti-CD3E immunotoxin UCHT1 (scFv)-DT
SKU: AGTO-G010D
Rating: 5 (1 reviews)

CAT#: AGTO-G010D

This immunotoxin was achieved by conjugating/fusing the Anti-CD3E scFv to Diphtheria toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to CD3ε positive T cells and the toxin enters cells by receptor-mediated endocytosis. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death. It was designed for treatment of T cell malignancies.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg

Normal Tissue Figure 2 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E

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Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
UCHT1

Molecule Class
scFv-DT

Toxin Description
Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
T cell malignancies

Target

Alternative Names
CD3E; CD3e molecule, epsilon (CD3-TCR complex); T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB390

Gene ID
916

UniProt ID
P07766

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-G010D.pdf

MSDS

MSDS - AGTO-G010D.pdf

COA

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