Mouse Anti-CD3E Recombinant Antibody (clone 2C11); Fab Fragment

Name: Mouse Anti-CD3E Recombinant Antibody (clone 2C11); Fab Fragment
SKU: PFBL-643
Rating: 5 (1 reviews)

CAT#: PFBL-643

Recombinant Mouse Antibody (2C11) Fab Fragment is specific to Mouse CD3epsilon.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg

Normal Tissue Figure 2 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E

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Specifications

Immunogen
Mouse CD3 antigen, epsilon polypeptide

Host Species
Mouse

Derivation
Mouse

Type
Mouse Fab

Specificity
mouse CD3epsilon

Species Reactivity
Mouse

Clone
2C11

Applications
WB, IHC, IF, FuncS

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CD3E; CD3 antigen, epsilon polypeptide; CD3; T3e; AI504783; CD3epsilon; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3Leu-4 epsilon chain

Gene ID
12501

UniProt ID
A6H6M1

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PFBL-643.pdf

MSDS

MSDS - PFBL-643.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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