Anti-CD3E immunotoxin UCHT1 (scFv)-RTA

Name: Anti-CD3E immunotoxin UCHT1 (scFv)-RTA
SKU: AGTO-G010R
Rating: 5 (1 reviews)

CAT#: AGTO-G010R

This immunotoxin was achieved by conjugating/fusing the Anti-CD3E scFv to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to CD3ε positive T cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of T cell malignancies.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg

Normal Tissue Figure 2 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E

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Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
UCHT1

Molecule Class
scFv-RTA

Toxin Description
Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
T cell malignancies

Target

Alternative Names
CD3E; CD3e molecule, epsilon (CD3-TCR complex); T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA

Gene ID
916

UniProt ID
P07766

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-G010R.pdf

MSDS

MSDS - AGTO-G010R.pdf

COA

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