Mouse Anti-CD3E Recombinant Antibody (clone UCHT1)
CAT#: DrMAB-988
Recombinant Mouse Antibody binds selectively to Mouse CD3E.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
❮
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Specifications
- Immunogen
- Human thymocytes/Sezary T cells
- Host Species
- Mouse
- Type
- Mouse IgG1 F(ab')2
- Specificity
- Antibody recognizes the 20 kD epsilon chain of the CD3 molecule complex.
- Species Reactivity
- Human
- Clone
- UCHT1
- Applications
- FC, IHC, WB, FuncS, IF
Product Property
- Purification
- Protein A purification
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- 1 mg/ml
- Buffer
- 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD3; CD3 epsilon; CD3e molecule, epsilon (CD3-TCR complex); TCRE; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4
- Gene ID
- 916
- UniProt ID
- P07766
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone UCHT1"
- CAT
- Product Name
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| AGTO-G010R | Anti-CD3E immunotoxin UCHT1 (scFv)-RTA | Cytotoxicity assay, Function study | |
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