What is the structural format of this antibody?

This product is an scFv-Fc Chimera (Human IgG1). It combines a single-chain variable fragment (scFv) specific for CD3E with an Fc region, enhancing its stability and ability to modulate T cell activation.

Human Anti-CD3E (clone NI-0401) scFv-Fc Chimera

CAT#: VS-0325-FY110

The anti-CD3E scFv-Fc antibody specifically binds to CD3ε, a subunit of the T cell receptor (TCR)complex. Its scFv-Fc format enhances its ability to modulate T cell activation and immune responses. This antibody is valuable for research on T cell signaling pathways and immune checkpoint mechanisms.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg

Normal Tissue Figure 2 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E

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Specifications

Host Species
Human

Type
Human IgG1, scFv-Fc

Specificity
Human CD3E

Species Reactivity
Human

Clone
NI-0401

Applications
ELISA

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Assay-dependent

Target

Alternative Names
CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3

Gene ID
916

UniProt ID
P07766

Long Name
CD3e Molecule

Cellular Localization
Cell membrane; Membrane

Post Translation Modifications
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Protein Refseq
NP_000724.1

Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

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Breast cancer biomarkers at key points during disease progression

Excellent scFv-Fc Chimera

We needed a reagent to modulate T cell activation, and this anti-CD3E scFv-Fc chimera was ideal. Clone NI-0401 bound specifically to the CD3 epsilon subunit. The chimera format provided the stability we needed for our extended incubation assays in immune checkpoint research.

High Specificity for CD3

This antibody showed excellent specificity for human CD3E in our ELISA tests. The purity was high, greater than 95%, which is critical for our signaling pathway studies. It is a valuable tool for investigating the T cell receptor complex and downstream signaling events.

Q&As

What is the structural format of this antibody?

A: This product is an scFv-Fc Chimera (Human IgG1). It combines a single-chain variable fragment (scFv) specific for CD3E with an Fc region, enhancing its stability and ability to modulate T cell activation.
What is the primary application for this antibody?

A: The primary listed application for this antibody is ELISA, and it is valuable for research on T cell signaling pathways and immune checkpoint mechanisms due to its specificity for the CD3 epsilon subunit.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0325-FY110.pdf

MSDS

MSDS - VS-0325-FY110.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Isotype Control

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Secondary Antibody

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