Mouse Anti-CNN3 Recombinant Antibody (VS3-FY2671) (CAT#: VS3-FY2671)
The mouse anti-CNN3 recombinant monoclonal antibody has been developed to target CNN3. The calculated molecular weight of the protein is 36.4 kDa. It shows capability of cross-reacting with human. This monoclonal antibody can be used in Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry, ELISA.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51237/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51237/if_selected.jpg
Figure 2 Placenta
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51237/125050_A_1_7_val_selected.jpg
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51237/125050_A_1_7_val_selected.jpg
Figure 3 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_A_7_5.jpg
Figure 4 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_A_4_6.jpg
Figure 5 Smooth muscle
(Smooth muscle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_B_4_7.jpg
(Smooth muscle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_B_4_7.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_B_7_5.jpg
Figure 7 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Microvilli Staining: High Intensity: Strong Fibroblasts Staining: Low Intensity: Weak Goblet cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Microvilli Staining: High Intensity: Strong Fibroblasts Staining: Low Intensity: Weak Goblet cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9849/27252_A_9_3.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117519-CNN3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117519-CNN3
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Calponin 3 of human
- Species Reactivity
- Human
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 36.4 kDa.
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Calponin 3; Calponin, Acidic Isoform; Calponin 3, Acidic; DJ639P13.2.2 (Acidic Calponin 3); Calponin-3
- Gene ID
- 1266
- Sequence Similarities
- Belongs to the calponin family.
- Post Translation Modifications
- Ubiquitination at Lys256
Modification sites at PhosphoSitePlus
Glycosylation from Gly
Glycosylation from GlyGen 2 sites, 5 N-linked glycans (1 site), 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047300436.1; XP_047300442.1; XP_054190200.1; XP_054190201.1; NP_001272984.1
- Function
- It is capable of binding to actin, calmodulin and tropomyosin.
The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4499 | PE-Mamu-A1*002:01/Human CNN3 (ASQKGMSVY) MHC Tetramer | FCM |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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