Rabbit Anti-CSDE1 Recombinant Antibody (clone R02-0E0) (CAT#: VS3-FY1989)

Clone R02-0E0 is a rabbit recombinant monoclonal antibody that specifically detects human CSDE1. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This CSDE1 antibody is guaranteed for western blot analysis of CSDE1/NRU in A549, HL-60, U2OS lysates.

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WB

Figure 1 Anti-CSDE1 Antibody (VS3-FY1989) in WB.

Figure 1 Anti-CSDE1 Antibody (VS3-FY1989) in WB.

The CSDE1/NRU expression in A549, HL-60, U2OS lysates were detected using the anti-CSDE1/NRU by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human CSDE1/NRU
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human CSDE1
  • Species Reactivity
  • Human
  • Clone
  • R02-0E0
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 89 kDa, while the observed molecular weight stands at 89 kDa.
  • Related Disease
  • Bulbar Polio and Persistent Generalized Lymphadenopathy

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Cold Shock Domain Containing E1; Cold Shock Domain Containing E1, RNA Binding; N-Ras Upstream Gene Protein; Upstream Of NRAS; D1S155E; UNR
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • Ubiquitination at Lys32, Lys166, Lys207, Lys239, Lys277, Lys295, Lys682, Lys729, and Lys758 (NX_O75534 [NX_O75534-1])
    Modification sites at PhosphoSitePlus (O75534)
    Glycosylation from GlyGen (O75534) 2 sites, 1 O-linked glycan (2 sites)
  • Function
  • RNA-binding protein involved in translationally coupled mRNA turnover.
    Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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