Mouse Anti-Diethylstilbestrol Recombinant Antibody (clone 30-3) (CAT#: FAMAB-0503WJ)
This product is a mouse monoclonal antibody that is specific for Diethylstilbestrol. This antibody can be used in a variety of applications, such as ELISA, WB.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Binding activity of soluble scFvs to DES-OVA by indirect ELISA.
The background (using wells coated with OVA) was subtracted from the absorbance of each DES conjugate. Control was the supernatant of the E. coli BL21 (DE3) without scFv gene.
Sun, Y., Ning, B., Liu, M., Gao, X., Fan, X., Liu, J., & Gao, Z. (2012). Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library. PLoS One, 7(3), e33186.
Figure 2 Competition assay for scFv to DES.
Different concentrations of free DES, ranging from 0.0005 to 50 µg/mL were used to inhibit the binding of the scFvs to DES-OVA conjugate. Control was the supernatant of E. coli the BL21 (DE3) with pTIG-TRX without scFv gene.
Sun, Y., Ning, B., Liu, M., Gao, X., Fan, X., Liu, J., & Gao, Z. (2012). Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library. PLoS One, 7(3), e33186.
SPR
Figure 3 The calculation of association rate constants by SPR.
30-3 scFv was appropriately diluted in PBS at five antibody concentrations.
Sun, Y., Ning, B., Liu, M., Gao, X., Fan, X., Liu, J., & Gao, Z. (2012). Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library. PLoS One, 7(3), e33186.
Specifications
- Host Species
- Mouse
- Derivation
- Ribosome Display Library
- Type
- Mouse IgG
- Specificity
- Diethylstilbestrol
- Clone
- 30-3
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Diethylstilbestrol; DES
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0504WJ | Mouse Anti-Diethylstilbestrol Recombinant Antibody (clone 37-1) | ELISA, WB | Mouse IgG |
| FAMAB-0505WJ | Mouse Anti-Diethylstilbestrol Recombinant Antibody (clone 75-1) | ELISA, WB | Mouse IgG |
| FAMAB-0506WJ | Mouse Anti-Diethylstilbestrol Recombinant Antibody (clone 108-5) | ELISA, WB | Mouse IgG |
| FAMAB-0507WJ | Mouse Anti-Diethylstilbestrol Recombinant Antibody (clone 146-1) | ELISA, WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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