Rabbit Anti-EEA1 Recombinant Antibody (clone R25-6Z-9)
CAT#: VS3-FY2112
Clone R25-6Z-9 is a recombinant monoclonal antibody that recognizes human EEA1 protein, a calculated weight of 162 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R25-6Z-9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18782/if_selected.jpg
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EEA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R25-6Z-9
- Applications
- Western Blot, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 162 kDa, while the observed molecular weight stands at 170kDa.
- Related Disease
- Subacute Cutaneous Lupus Erythematosus and Cutaneous Lupus Erythematosus
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- EEA1; MST105; MSTP105; ZFYVE2; early endosome antigen 1
- Gene ID
- 8411
- UniProt ID
- Q15075
- Cellular Localization
- Cytoplasm, Endosome, Membrane
- Post Translation Modifications
- Ubiquitination at Lys58 (NX_Q15075 [NX_Q15075-1])
Modification sites at PhosphoSitePlus (Q15075)
Glycosylation from GlyGen (Q15075) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_011537116.1; XP_054229406.1; XP_054229407.1; NP_003557.3
- Function
- Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2112. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "EEA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1408z | Mouse Anti-EEA1 Recombinant Antibody (clone 34C5) | WB, ICC, IF, IHC | Mouse IgG1 |
| MOB-2311CT | Recombinant Mouse anti-Human EEA1 Monoclonal antibody (FQS2967) | ICC, IF | |
| MRO-0517-CN | Mouse Anti-EEA1 Recombinant Antibody (clone B0-A8) | WB, IF, IHC | Mouse IgG1 |
| MRO-1831-CN | Rabbit Anti-EEA1 Polyclonal Antibody (MRO-1831-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0182MZ | Chicken Anti-EEA1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1093 | Hi-Affi™ Rabbit Anti-EEA1 Recombinant Antibody (clone DS1093AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2158 | Anti-EEA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2159 | Anti-Mouse EEA1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, WB, SPR, IF, EM
Application: FC, Cyt, Stim, PP, Agonist
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
Application: FC, Cyt, Stim, PP, Agonist
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
