Recombinant Mouse Anti-EF Antibody (9F3)
CAT#: FAMAB-0212-CN
This is a monoclonal antibody against Edema factor (EF), a Bacillus anthracis component of an anthrax toxin that functions as an adenylate cyclase. The antibody 9F3 could neutralize EF and has a relatively low binding constant despite its neutralizing capacity.
Specifications
- Immunogen
- EF protein
- Host Species
- Mouse
- Type
- Mouse IgM
- Specificity
- Bacillus anthracis EF
- Species Reactivity
- Bacillus anthracis
- Clone
- 9F3
- Applications
- ELISA, BL, IEM
- Related Disease
- Anthrax
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Blocking and Immuno-Electron Microscopy.
Enzyme-Linked Immunosorbent Assay: A MAb-based EF capture ELISA was developed to detect EF in serum or organ homogenates from infected mice. Briefly, 96-well microtiter plates were coated with 1 μg/ml of goat anti-mouse IgM unlabeled in PBS, blocked with 1% BSA–PBS, and then incubated with a murine MAb to EF, IgM MAb 9F3, which functioned as the capture Ab. Next, we added rEF (positive control) and either serum or organ homogenates from infected mice or organ homogenates spiked with rEF (1μg/ml) at different dilutions. EF captured by the IgM was detected with the IgG1 MAb 21B9. Microtiter plates were then incubated with isotype-specific GAM IgG conjugated to AP and developed with p-nitrophenyl phosphate (PNPP), and absorbance at 405 nm was measured as described above. All incubations were carried at 37°C for 1 h.
Target
- Alternative Names
- EF
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 9F3"
- CAT
- Product Name
See other products for "EF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0213-CN | Recombinant Mouse Anti-EF Antibody (8F3) | ELISA | Mouse IgG1 |
| FAMAB-0214-CN | Recombinant Mouse Anti-EF Antibody (21B9) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0212-CN-S(P) | Recombinant Mouse Anti-EF Antibody scFv Fragment (9F3) | ELISA, BL | Mouse scFv |
| FAMAB-0213-CN-S(P) | Recombinant Mouse Anti-EF Antibody scFv Fragment (8F3) | ELISA | Mouse scFv |
| FAMAB-0214-CN-S(P) | Recombinant Mouse Anti-EF Antibody scFv Fragment (21B9) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0212-CN-F(E) | Recombinant Mouse Anti-EF Antibody Fab Fragment (9F3) | ELISA, BL | Mouse Fab |
| FAMAB-0213-CN-F(E) | Recombinant Mouse Anti-EF Antibody Fab Fragment (8F3) | ELISA | Mouse Fab |
| FAMAB-0214-CN-F(E) | Recombinant Mouse Anti-EF Antibody Fab Fragment (21B9) | ELISA | Mouse Fab |
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