Recombinant Mouse Anti-EF Antibody (9F3) (CAT#: FAMAB-0212-CN)

• Immunogen
• EF protein

• Host Species
• Mouse

• Type
• Mouse IgM

• Specificity
• Bacillus anthracis EF

• Species Reactivity
• Bacillus anthracis

• Clone
• 9F3

• Applications
• ELISA, BL, IEM

• Related Disease
• Anthrax

• Storage
• Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Blocking and Immuno-Electron Microscopy.
  Enzyme-Linked Immunosorbent Assay: A MAb-based EF capture ELISA was developed to detect EF in serum or organ homogenates from infected mice. Briefly, 96-well microtiter plates were coated with 1 μg/ml of goat anti-mouse IgM unlabeled in PBS, blocked with 1% BSA–PBS, and then incubated with a murine MAb to EF, IgM MAb 9F3, which functioned as the capture Ab. Next, we added rEF (positive control) and either serum or organ homogenates from infected mice or organ homogenates spiked with rEF (1μg/ml) at different dilutions. EF captured by the IgM was detected with the IgG1 MAb 21B9. Microtiter plates were then incubated with isotype-specific GAM IgG conjugated to AP and developed with p-nitrophenyl phosphate (PNPP), and absorbance at 405 nm was measured as described above. All incubations were carried at 37°C for 1 h.

• Alternative Names
• EF

• FAMAB-0212-CN-S(P)
• Recombinant Mouse Anti-EF Antibody scFv Fragment (9F3)

• FAMAB-0212-CN-F(E)
• Recombinant Mouse Anti-EF Antibody Fab Fragment (9F3)

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