Recombinant Chicken Anti-F. verticillioides Antibody (FvCA3) (CAT#: FAMAB-0142-CN)
The mAb FvCA3 is a highly sensitive monoclonal antibody against Fusarium verticillioides, the primary causal agent of Fusarium ear and kernel rot in maize, producing fumonisin mycotoxins that are toxic to humans and domestic animals.
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WB
Figure 1 Immunoblot of soluble scFv antibodies extracted from periplasm in Escherichia coli.
The four selected scFv antibodies were induced for expression by IPTG in E. coli. Periplasmic proteins were separated by 12% SDS-PAGE and blotted onto a nitrocellulose membrane. After blocking with 5% (w/v) skimmed milk, the membrane was incubated with a monoclonal anti-His antibody and detected with AP-conjugated goat anti-mouse IgG antibody. The colorimetric reaction was carried out by adding BCIP/NBT substrate. M, protein molecular weight standards. Lanes 1 to 4, FvCA1, FvCA2, FvCA3 and FvCA4 antibodies.
Hu, Z. Q., Liu, J. L., Li, H. P., Xing, S., Xue, S., Zhang, J. B., ... & Liao, Y. C. (2012). Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International journal of molecular sciences, 13(6), 7038-7056.
ELISA
Figure 2 Expression ELISA against CWPs of Fusarium verticillioides with bacterially expressed soluble scFv antibodies.
Four selected scFv antibodies were analyzed in the presence and absence (control) of the antigens, and the y axis represents the OD405nm values minus control values. Values represent mean ± SD of triplicate assays. Letters A, B and C above the column diagram represent a significant difference at p < 0.01.
Hu, Z. Q., Liu, J. L., Li, H. P., Xing, S., Xue, S., Zhang, J. B., ... & Liao, Y. C. (2012). Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International journal of molecular sciences, 13(6), 7038-7056.
Specifications
- Immunogen
- The mycelia collected from a fungus strain of F. verticillioides, wh1-2, isolated from the rot kernels of maize, collected from the seriously epidemic area of maize ear rot in Wuhan, China
- Host Species
- Chicken
- Derivation
- Phage display library
- Type
- Chicken IgY
- Specificity
- Fusarium verticillioides F. verticillioides
- Species Reactivity
- Fusarium verticillioides
- Clone
- FvCA3
- Applications
- ELISA, IF, WB
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Immunofluorescence and Western Blot.
Target
- Alternative Names
- F. verticillioides
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
ScFv Antibodies
Fab Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0143-CN | Recombinant Chicken Anti-F. verticillioides Antibody (FvCA4) | ELISA, IF, WB | Chicken IgY |
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For Research Use Only. Not For Clinical Use.
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