Recombinant Chicken Anti-F. verticillioides Antibody (FvCA3) (CAT#: FAMAB-0142-CN)

Figure 1 Immunoblot of soluble scFv antibodies extracted from periplasm in Escherichia coli.

The four selected scFv antibodies were induced for expression by IPTG in E. coli. Periplasmic proteins were separated by 12% SDS-PAGE and blotted onto a nitrocellulose membrane. After blocking with 5% (w/v) skimmed milk, the membrane was incubated with a monoclonal anti-His antibody and detected with AP-conjugated goat anti-mouse IgG antibody. The colorimetric reaction was carried out by adding BCIP/NBT substrate. M, protein molecular weight standards. Lanes 1 to 4, FvCA1, FvCA2, FvCA3 and FvCA4 antibodies.

Hu, Z. Q., Liu, J. L., Li, H. P., Xing, S., Xue, S., Zhang, J. B., ... & Liao, Y. C. (2012). Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International journal of molecular sciences, 13(6), 7038-7056.

Figure 2 Expression ELISA against CWPs of Fusarium verticillioides with bacterially expressed soluble scFv antibodies.

Four selected scFv antibodies were analyzed in the presence and absence (control) of the antigens, and the y axis represents the OD405nm values minus control values. Values represent mean ± SD of triplicate assays. Letters A, B and C above the column diagram represent a significant difference at p < 0.01.

Hu, Z. Q., Liu, J. L., Li, H. P., Xing, S., Xue, S., Zhang, J. B., ... & Liao, Y. C. (2012). Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International journal of molecular sciences, 13(6), 7038-7056.