This product is a Mouse antibody that recognizes Human Fc P329G LALA.
Figure 1 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in SDS-PAGE
SDS-PAGE analysis of PABJ-0115 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 2 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
Figure 3 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in WB
Western blot analysis was performed by loading recombinant human IgG1 with Fc P329G LALA onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human Fc P329G LALA Antibody (PABJ-0115) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Figure 4 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in ELISA
ELISA analysis of PABJ-0115 was performed by coating with recombinant human IgG1 with Fc P329G LALA. Then blocked with BSA and incubated with Anti-Human Fc P329G LALA Antibody (PABJ-0115) at a starting concentration of 2.5 ng/mL. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in DB
Dot Blot analysis of PABJ-0115 was performed by coating with recombinant human IgG1 with Fc P329G LALA.
PABJ-0115 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0115, RRID: AB_3111605)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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