Mouse Anti-HBx Recombinant Antibody (clone 2A7)
CAT#: VS-0723-CJ30
This mouse antibody 2A7 targets a linear epitope encompassing amino acids 89 to 96 of HBx. Anti-HBx mAb 2A7 broadly reacts with native and denatured HBx encoded by multiple HBV strains.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Hepatitis B virus (HBV) X protein (HBx)
- Species Reactivity
- HBV
- Clone
- 2A7
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Hepatitis
Product Property
- Purification
- Protein G purified
- Purity
- >95%
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The reactivity and specificity of anti-HBx antibody 2A7 was confirmed in both Western blot and immunofluorescence. 2A7 specifically recognizes all four HBx proteins (BPS, B6, B200, WT HBV isolates) expressed in HEK293T cells in both Western blot and immunofluorescence. For pull-down assay, cell lysates were first mixed with or without 2A7 (2 μg/ml).
Target
- Alternative Names
- Hepatitis B virus X protein
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Customer Review
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Effective in Western Blot
I used the Mouse Anti-HBx Recombinant Antibody (clone 2A7) for Western blotting, and the results were crisp and clear. The targeted protein band was distinct, making data interpretation straightforward.
Flow Cytometry Compatibility
The Mouse Anti-HBx Recombinant Antibody (clone 2A7) performed excellently in flow cytometry assays. Staining was specific, and the protocol was straightforward to follow, providing clear results.
Minimal Background Noise
Using this antibody resulted in minimal background noise in my Western blots, making it easier to interpret specific HBx signals.
Q&As
-
What is the specificity of Mouse Anti-HBx Recombinant Antibody (clone 2A7)?
A: The HBx protein of the Hepatitis B virus is specifically bound by the Mouse Anti-HBx Recombinant Antibody (clone 2A7), enabling focused detection and research uses.
-
How can I validate the performance of this antibody in Western Blotting?
A: Make sure the band fits the expected molecular weight by validating the performance using positive HBx-protein controls and comparing the outcomes with previously published datasets or literature.
-
How can the antibody's binding be confirmed in flow cytometry?
A: To verify specific binding, use isotype and known positive controls. Calibration beads for flow cytometers can also be used to verify fluorescent conjugation.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "HBx"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-650CQ | Recombinant Mouse Anti-HBx Antibody (VC99) | ELISA, WB, ICC | Mouse antibody |
| MRO-651CQ | Recombinant Mouse Anti-HBx Antibody (VC220) | ELISA, WB, ICC | Mouse antibody |
| MRO-652CQ | Recombinant Mouse Anti-HBx Antibody (X36C) | ELISA, WB, IP | Mouse antibody |
| MRO-653CQ | Recombinant Mouse Anti-HBx Antibody (3F6-G10) | ELISA, IHC, WB | Mouse antibody |
| HPAB-N0131-YC | Recombinant Mouse Anti-HBx Antibody (KY-02) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0131-YC-S(P) | Recombinant Mouse Anti-HBx Antibody scFv Fragment (KY-02) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0131-YC-F(E) | Recombinant Mouse Anti-HBx Antibody Fab Fragment (KY-02) | ELISA, WB | Mouse Fab |
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