Mouse Anti-HDAC5 Recombinant Antibody (VS3-CJ28)
CAT#: VS3-CJ28
This product is a mouse antibody that recognizes HDAC5.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19400/42719_B_8_6_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_B_8_5.jpg
Normal Tissue
Figure 5 Cerebellum
Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_B_9_8.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30991/90543_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108840-HDAC5
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Specifications
- Immunogen
- Recombinant fragment of human HDAC5 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HDAC5
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- HD5; NY-CO-9
- Gene ID
- 10014
- UniProt ID
- Q9UQL6
- Sequence Similarities
- Belongs to the histone deacetylase family. HD type 2 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).
- Protein Refseq
- NP_001015053.1; NP_005465.2
- Function
- Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed:28167758).
In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed:28167758).
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Related Diseases
Alcoholism
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "HDAC5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2257z | Mouse Anti-HDAC5 Recombinant Antibody (clone 31D4) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| MOB-1871MZ | Recombinant Mouse Anti-Human HDAC5 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1003 | Anti-HDAC5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3099 | Anti-Human HDAC5 Immunohistochemistry Kit | IHC |
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