Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 27A8) (CAT#: MRO-4318CQ)
Recombinant mouse antibody to Tat. MAb 27A8 recognized a linear epitope at Tat C-terminal domain, conserved only between Tats Oyi and HxB2 (identity 87%), but not in other Tat variants. MAb 27A8 bound only Tats Oyi and HxB2 with high affinities.
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ELISA
Figure 1 mAb 7G12 recognizes a 3D epitope.
p< 0.01 between folded Tats and either denatured proteins or peptides.
Mediouni, S., Watkins, J. D., Pierres, M., Bole, A., Loret, E. P., & Baillat, G. (2012). A monoclonal antibody directed against a conformational epitope of the HIV-1 trans-activator (Tat) protein neutralizes cross-clade. Journal of Biological Chemistry, 287(15), 11942-11950.
WB
Figure 2 Recognition of Tat Oyi on immunoblot analysis by mAbs 27A8 (1:5000).
Shown is a representative assay of three
experiments.
Mediouni, S., Watkins, J. D., Pierres, M., Bole, A., Loret, E. P., & Baillat, G. (2012). A monoclonal antibody directed against a conformational epitope of the HIV-1 trans-activator (Tat) protein neutralizes cross-clade. Journal of Biological Chemistry, 287(15), 11942-11950.
Specifications
- Immunogen
- A clade B Tat variant, named Tat Oyi
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Recognizes HIV-1 Tat
- Species Reactivity
- HIV-1
- Clone
- 27A8
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Tat antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- TAT; p14; Tat; Transactivating regulatory protein; HIV-1
- Gene ID
- 155871
- UniProt ID
- P04608
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
IgM Antibodies
ScFv Antibodies
Fab Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-568 | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 11H6H1) | ELISA, Neut | Mouse IgG |
| MRO-1840CQ | Mouse Anti-HIV-1 Tat Recombinant Antibody (MRO-1840CQ) | ICC, WB | Mouse IgG1 |
| MRO-4310CQ | Human Anti-HIV-1 Tat Recombinant Antibody (clone B1E3) | Neut, Inhib, WB | Human IgG1, κ |
| MRO-4311CQ | Human Anti-HIV-1 Tat Recombinant Antibody (clone G1) | WB, IP, Neut | Human IgG1, κ |
| MRO-4312CQ | Human Anti-HIV-1 Tat Recombinant Antibody (clone G2) | WB, FC | Human IgG1, κ |
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