Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 27A8) (CAT#: MRO-4318CQ)
Recombinant mouse antibody to Tat. MAb 27A8 recognized a linear epitope at Tat C-terminal domain, conserved only between Tats Oyi and HxB2 (identity 87%), but not in other Tat variants. MAb 27A8 bound only Tats Oyi and HxB2 with high affinities.
Specific Inquiry
ELISA
Figure 1 mAb 7G12 recognizes a 3D epitope.
p< 0.01 between folded Tats and either denatured proteins or peptides.
Mediouni, S., Watkins, J. D., Pierres, M., Bole, A., Loret, E. P., & Baillat, G. (2012). A monoclonal antibody directed against a conformational epitope of the HIV-1 trans-activator (Tat) protein neutralizes cross-clade. Journal of Biological Chemistry, 287(15), 11942-11950.
WB
Figure 2 Recognition of Tat Oyi on immunoblot analysis by mAbs 27A8 (1:5000).
Shown is a representative assay of three
experiments.
Mediouni, S., Watkins, J. D., Pierres, M., Bole, A., Loret, E. P., & Baillat, G. (2012). A monoclonal antibody directed against a conformational epitope of the HIV-1 trans-activator (Tat) protein neutralizes cross-clade. Journal of Biological Chemistry, 287(15), 11942-11950.
Specifications
- Immunogen
- A clade B Tat variant, named Tat Oyi
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Recognizes HIV-1 Tat
- Species Reactivity
- HIV-1
- Clone
- 27A8
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Tat antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- TAT; p14; Tat; Transactivating regulatory protein; HIV-1
- Gene ID
- 155871
- UniProt ID
- P04608
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-565 | Mouse Anti-HIV-1 Tat Recombinant Antibody Fab Fragment (PFBL-565) | ELISA, Neut | Mouse Fab |
| MRO-4310CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone B1E3); Fab Fragment | ELISA, Neut | Human Fab |
| MRO-4311CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone G1); Fab Fragment | WB, IP, Neut | Human Fab |
| MRO-4312CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone G2); Fab Fragment | WB, IP, Neut | Human Fab |
| MRO-4313CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone J1); Fab Fragment | WB, IP, Neut | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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