Human Anti-HIV-1 Vif Recombinant Antibody (clone J4) (CAT#: MRO-4329CQ)
Recombinant Humanized Antibody to Vif. J4 bound strongly to Vif protein.
Specific Inquiry
ELISA
Figure 1 Relative binding affinities of anti-Vif Fab antibody fragments.
Isolated anti-Vif Fabs in a total of 96 clones were expressed
and used for binding to 200 ng of Vif protein.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
WB
Figure 2 Expression and relative binding affinities of anti-Vif Fab and scFv.
TOP 10 F bacteria expressing anti-Vif 4 BL scFv (lanes 1
and 2), anti-Vif 4B scFv (lanes 3 and 4), and Fab J4 (lanes 5 and 6) at 2 and 18 h, respectively, by induction with 0.5 M isopropyl-β-D thiogalactopyranoside at 25 °C.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
IF
Figure 3 Detection of antigens by immunofluorescence.
The detection of Vif protein expressed in HeLa cells transfected with pSVLvif.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
Neut
Figure 4 Neutralization of Vif function in a trans-complementation assay.
The values shown by the black and white bars represent
the percentages of replication complementation in Jurkat and H9 cells,
respectively.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
Inhib
Figure 5 Cell-specific inhibition of HIV-1 replication in presence of anti-Vif scFv.
Replication of HIV-encoding anti-Vif scFv anti-thyroglobulin was assessed in permissive and nonpermissive cells.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Recognizes HIV-1 Vif
- Species Reactivity
- HIV-1
- Clone
- J4
- Applications
- WB, ELISA, Neut, IF, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Tat antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Neutralization, Inhibition, Immunofluorescence.
Target
- Alternative Names
- Human immunodeficiency virus 1; HIV-1; Viral infectivity factor; VIF; Vif
- Gene ID
- 155459
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone J4"
See other products for "HIV-1 Vif"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1043CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone 319) | WB, IF | Mouse IgG1 |
| MRO-1044CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone CSK-04) | IF, WB | Mouse IgG1 |
| MRO-1045CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone TG001) | ELISA | Mouse IgG2b, κ |
| MRO-1046CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone 564) | IP | Mouse IgG1 |
| MRO-4330CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone TG002) | Inhib | Mouse IgG |
MHC Tetramer for Virology
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC254 | PE-A*02:01/HIV-1 Vif (GLADQLIHL) MHC Tetramer | FCM | |
| MHC-LC255 | APC-A*02:01/HIV-1 Vif (GLADQLIHL) MHC Tetramer | FCM | |
| MHC-LC256 | BV421-A*02:01/HIV-1 Vif (GLADQLIHL) MHC Tetramer | FCM |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4329CQ-S(P) | Human Anti-HIV-1 Vif Recombinant Antibody (clone J4); scFv Fragment | ELISA, IF, WB | Humanized scFv |
| MRO-4330CQ-S(P) | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone TG002); scFv Fragment | ELISA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4329CQ-F(E) | Human Anti-HIV-1 Vif Recombinant Antibody (clone J4); Fab Fragment | ELISA, IF, WB | Humanized Fab |
| MRO-4330CQ-F(E) | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone TG002); Fab Fragment | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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