Human Anti-HIV-1 Vif Recombinant Antibody (clone J4) (CAT#: MRO-4329CQ)
Recombinant Humanized Antibody to Vif. J4 bound strongly to Vif protein.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Relative binding affinities of anti-Vif Fab antibody fragments.
Isolated anti-Vif Fabs in a total of 96 clones were expressed
and used for binding to 200 ng of Vif protein.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
WB
Figure 2 Expression and relative binding affinities of anti-Vif Fab and scFv.
TOP 10 F bacteria expressing anti-Vif 4 BL scFv (lanes 1
and 2), anti-Vif 4B scFv (lanes 3 and 4), and Fab J4 (lanes 5 and 6) at 2 and 18 h, respectively, by induction with 0.5 M isopropyl-β-D thiogalactopyranoside at 25 °C.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
IF
Figure 3 Detection of antigens by immunofluorescence.
The detection of Vif protein expressed in HeLa cells transfected with pSVLvif.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
Neut
Figure 4 Neutralization of Vif function in a trans-complementation assay.
The values shown by the black and white bars represent
the percentages of replication complementation in Jurkat and H9 cells,
respectively.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
Inhib
Figure 5 Cell-specific inhibition of HIV-1 replication in presence of anti-Vif scFv.
Replication of HIV-encoding anti-Vif scFv anti-thyroglobulin was assessed in permissive and nonpermissive cells.
Goncalves, J., Silva, F., Freitas-Vieira, A., Santa-Marta, M., Malhó, R., Yang, X., ... & Barbas, C. (2002). Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. Journal of Biological Chemistry, 277(35), 32036-32045.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Recognizes HIV-1 Vif
- Species Reactivity
- HIV-1
- Clone
- J4
- Applications
- WB, ELISA, Neut, IF, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Tat antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Neutralization, Inhibition, Immunofluorescence.
Target
- Alternative Names
- Human immunodeficiency virus 1; HIV-1; Viral infectivity factor; VIF; Vif
- Gene ID
- 155459
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone J4"
See other products for "HIV-1 Vif"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
MHC Tetramer Products for Virology
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1043CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone 319) | WB, IF | Mouse IgG1 |
| MRO-1044CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (MRO-1044CQ) | IF, WB | Mouse IgG1 |
| MRO-1045CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (MRO-1045CQ) | ELISA | Mouse IgG2b, κ |
| MRO-1046CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (MRO-1046CQ) | IP | Mouse IgG1 |
| MRO-4330CQ | Mouse Anti-HIV-1 Vif Recombinant Antibody (clone TG002) | Inhib | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for MRO-4329CQ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
-1.png)
Application: IB, ELISA, FC, FuncS
Application: WB, IHC, ELISA, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
Application: ELISA, WB, Microarray, Block
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
