Mouse Anti-HNRNPM Recombinant Antibody (clone 7E2D6)
CAT#: VS7-0425-WR453
This product is a mouse IgG1 antibody that binds to human HNRNPM. The antibody 7E2D6 recognizes specific amino acid domain of human HNRNPM (AA: 17-161). It can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16113/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_A_1_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24344/54866_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099783-HNRNPM
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Specifications
- Immunogen
- Recombinant human HNRNPM (AA: 17-161) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HNRNPM
- Species Reactivity
- Human
- Clone
- 7E2D6
- Molecule Class
- Heterogeneous Nuclear Ribonucleoproteins (Hnrnps)
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M
- Gene ID
- 4670
- UniProt ID
- P52272
- Long Name
- Heterogeneous Nuclear Ribonucleoprotein M
- Cellular Localization
- Nucleus; Spliceosome
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001284347.1
- Function
- Ribonucleoprotein; RNA-binding
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "HNRNPM"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2377MZ | Recombinant Mouse Anti-Human HNRNPM Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| VS3-FY694 | Recombinant Rabbit Anti-HNRNPM Antibody (clone R06-3B1) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR454 | Mouse Anti-HNRNPM Recombinant Antibody (clone 5G6C11) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1649 | Rabbit Anti-HNRNPM Recombinant Antibody (clone DS1649AB) | IHC-P, WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1049 | Anti-HNRNPM Immunohistochemistry Kit | IHC | |
| VS-0525-XY3244 | Anti-Mouse HNRNPM Immunohistochemistry Kit | IHC | |
| VS-0525-XY3243 | Anti-Human HNRNPM Immunohistochemistry Kit | IHC | |
| VS-0525-XY3245 | Anti-Rat HNRNPM Immunohistochemistry Kit | IHC |
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