Recombinant Mouse Anti-HPV16 L2 Antibody (14H6)
CAT#: HPAB-AP368-YC
Provided is a monoclonal antibody 14H6 that capable of binding to human papillomavirus (HPV) L2 protein.
Published Data
Neut
Figure 1 Screening for L2 neutralizing monoclonal antibodies (mAbs) using HPV16L2 C50 antigen.
Neutralization geometric mean titers (GMTs) of mAbs 14H6, 15H5, 15E4, 6H8 mAbs were plotted on the log-scaled y-axis against various genotypes of HPV-PsVs on the x-axis. The bar graph was prepared using Prism GraphPad 5.0. All mAb samples were adjusted to an initial concentration of 1.0 mg/ml prior to two-fold serial dilutions. Flow cytometry was used to detect the number of HPV-pseudovirus-infected enhanced green fluorescent protein (EGFP)-expressing cells.
Wang, D., Li, Z., Xiao, J., Wang, J., Zhang, L., Liu, Y., ... & Yu, H. (2015). Identification of broad-genotype HPV L2 neutralization site for pan-HPV vaccine development by a cross-neutralizing antibody. PLoS One, 10(4).
ELISA
Figure 2 Characterization of 14H6a–f chimeras.
EC50 test by ELISA using a serial dilution of mAb 14H6. The reaction curves vs. the concentration of mAb 14H6 were fitted to a sigmoid trend and the EC50 values were calculated in terms of the half points (shown in the brackets). Detection in western blotting and the EC50 results indicate that all chimeric proteins present mAb 14H6 with similar binding capabilities.
Wang, D., Li, Z., Xiao, J., Wang, J., Zhang, L., Liu, Y., ... & Yu, H. (2015). Identification of broad-genotype HPV L2 neutralization site for pan-HPV vaccine development by a cross-neutralizing antibody. PLoS One, 10(4).
EM
Figure 3 Immunoelectron microscopy of recombinant bacteria.
EM images of ER0808 cells, ER0808-prL8L2 cells, ER2566-pF-L2 cells and ER2566-pC-L2 cells as indicated by gold-conjugated 14H6 (set b, top panel) at ×49,000 magnification. For clarity, close-up views of the top panels are shown; red boxes indicate the magnified regions. The curve of some images below is adjusted to better visualize the gold particles by Photoshop software.
Chen, T., Wang, K., Chi, X., Zhou, L., Li, J., Liu, L., ... & Zhang, J. (2019). Construction of a bacterial surface display system based on outer membrane protein F. Microbial cell factories, 18(1), 70.
WB
Figure 4 Identifcation of fusion protein expression and purifcation.
SDS-PAGE and western blotting of total proteins (TP) and OMPs prepared from equal biomass of ER0808 and a ER0808-prL8HB, ER2566(pF-HB) and ER2566(pC-HB) or b ER0808-prL8L2, ER2566(pF-L2) and ER2566(pC-L2). Black arrows indicate the fusion proteins. Samples (10 µL) with an equivalent amounts of cell lysate, were loaded.
Chen, T., Wang, K., Chi, X., Zhou, L., Li, J., Liu, L., ... & Zhang, J. (2019). Construction of a bacterial surface display system based on outer membrane protein F. Microbial cell factories, 18(1), 70.
FC
Figure 5 Flow cytometry of recombinant bacteria.
E6F6 epitope display strains (a) and E6F6 epitope display strains (b) were analyzed with E6F6 or 14H6 as the primary antibodies and FITC-conjugated secondary antibodies in fow cytometer. ER2566 and ER0808 served as negative control. The results were illustrated as histogram mode and positive cell ratios were indicated.
Chen, T., Wang, K., Chi, X., Zhou, L., Li, J., Liu, L., ... & Zhang, J. (2019). Construction of a bacterial surface display system based on outer membrane protein F. Microbial cell factories, 18(1), 70.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- HPV16
- Species Reactivity
- HPV16
- Clone
- 14H6
- Applications
- Neut, ELISA, EM, WB, FC
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Applications
- Application Notes
- The HPV16 L2 antibody has been reported in applications of Neutralization, Enzyme-linked Immunosorbent Assay, Electron Microscopy, Western Blot, Flow Cytometry.
Target
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Enhanced Sensitivity
The recombinant mouse anti-HPV16 L2 antibody by Creative Biolabs offers enhanced sensitivity, crucial for precise detection in virology research. This specificity significantly improves the accuracy of experimental results.
Reliable in Neutralization Assays
This antibody demonstrates exceptional reliability in neutralization assays, providing consistent and reproducible results. Its high specificity ensures accurate identification of HPV16 L2, surpassing other similar products.
Effective in ELISA Applications
In ELISA applications, the recombinant mouse anti-HPV16 L2 antibody is highly effective. It delivers precise quantification of L2 proteins, outperforming many similar antibodies in accuracy and reliability.
Q&As
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What is the optimal protocol for immunoprecipitation using mouse anti-HPV16 L2 recombinant antibody?
A: For immunoprecipitation, incubate cell lysates with the mouse anti-HPV16 L2 recombinant antibody to form an antibody-antigen complex. Use protein A/G agarose beads to capture the complex, wash thoroughly to remove non-specific proteins, and then elute the bound antigen for further analysis.
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How can the mouse anti-HPV16 L2 recombinant antibody be used in immunohistochemistry?
A: In immunohistochemistry, deparaffinize and rehydrate tissue sections, perform antigen retrieval, and block non-specific binding with a suitable blocking buffer. Incubate the sections with the antibody, followed by a secondary antibody conjugated to a detection enzyme or fluorophore, and visualize using a chromogenic substrate or fluorescence microscopy.
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What steps are necessary to prepare samples for Western blot analysis using mouse anti-HPV16 L2 recombinant antibody?
A: Prepare samples by lysing cells or tissues in an appropriate buffer, separate proteins by SDS-PAGE, and transfer them to a PVDF or nitrocellulose membrane. Block the membrane, incubate with the antibody, wash, and then incubate with a secondary antibody. Detect the signal using a chemiluminescent or colorimetric substrate.
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How should the mouse anti-HPV16 L2 recombinant antibody be conjugated to a fluorophore for flow cytometry?
A: Conjugate the antibody to a fluorophore by following a labeling kit's instructions. Typically, this involves mixing the antibody with the fluorophore reagent, incubating, and purifying the conjugated antibody to remove unbound fluorophore. Use the conjugated antibody in flow cytometry to detect HPV16 L2 expression.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 14H6"
- CAT
- Product Name
See other products for "HPV16 L2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-544LC | Mouse Anti-HPV16 L2 Recombinant Antibody (MRO-544LC) | ELISA | Mouse IgG1, κ |
| MRO-544LC-F(E) | Mouse Anti-HPV16 L2 Recombinant Antibody; Fab Fragment (MRO-544LC-F(E)) | ELISA | Mouse Fab |
| MRO-545LC-F(E) | Mouse Anti-HPV16 L2 Recombinant Antibody; Fab Fragment (MRO-545LC-F(E)) | ELISA | Mouse Fab |
| MRO-561LC-F(E) | Anti-HPV16 L2 Recombinant Antibody Fab Fragment (4G1) | ELISA | Mouse antibody |
| MRO-562LC-F(E) | Anti-HPV16 L2 Recombinant Antibody Fab Fragment (4G5) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1904CQ | Recombinant Mouse Anti-HPV-16 L2 Antibody (VC267) | WB, IF | Mouse antibody |
| MRO-1905CQ | Recombinant Mouse Anti-HPV-16 L2 Antibody (VC268) | WB, IF | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP368-YC-S(P) | Recombinant Mouse Anti-HPV16 L2 Antibody scFv Fragment (14H6) | ELISA, Neut, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP368-YC-F(E) | Recombinant Mouse Anti-HPV16 L2 Antibody Fab Fragment (14H6) | ELISA, Neut, WB | Mouse Fab |
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