Mouse Anti-HPV58 L1 Recombinant Antibody (VS-0724-FY37)
CAT#: VS-0724-FY37
This is a mouse IgG2a monoclonal antibody that binds to HPV58 L1. 5G9 recognizes amino acids R135, Y136, and A138 form the major interactions involving HPV58. The binding affinity EC₅₀ for clone 5G9 is 48.93 ng/mL. 2H3 recognizes epitopes involving the BC, EF, FG, and HI loops of the HPV58 capsid and exhibits the highest binding and neutralization efficiency. The antibody 2H3 has the highest inhibition (87%) when recognizing an immunodominant epitope. The IC₅₀ of clone 2H3 is 3.3 ng/mL.
Published Data
ELISA
Figure 1. Recombinant Mouse Anti-HPV58 L1 Antibody (5G9) in ELISA.
Clustering analysis of 15 nAbs directed against HPV58 VLPs based on competitive ELISA (cELISA). The 15 nAbs were clustered into 3 groups (C1 [blue], C2 [red], and C3 [green]) (left) using SPSS based on their cELISA profiles (right). The heat map of the cELISA data is depicted according to the inhibition rate (percent). Parameters are colored continuously from green to red (0, green; 50, black; 100, red).
1. He, Maozhou, et al. "Structural basis for the shared neutralization mechanism of three classes of human papillomavirus type 58 antibodies with disparate modes of binding." Journal of Virology 95.7 (2021): 10-1128. 2. under Open Access license cc BY 4.0. without modification.
IF
Figure 2. Recombinant Mouse Anti-HPV58 L1 Antibody (5G9 and 2H3) in IF.
Attachment assay for HPV58 nAbs to the ECM and cell surface. PsV58 (green) was preincubated with either BSA (control group) or 5G9, as indicated, and then added to the ECM or the cell surface. Laminin-5 (red) stain was used for the ECM in an ECM binding assay, and phalloidin (red) was used for activity in the cell-surface binding assay. Green fluorescent PsV58 was preincubated with either BSA (control group) or 2H3, as specified, before being added to the ECM or cell surface. A red stain, laminin-5, was used to visualize binding to the ECM, while phalloidin, another red stain, was used to assess binding to the cell surface. DAPI fluorescence (blue) was used to identify the cell nuclei.
1. He, Maozhou, et al. "Structural basis for the shared neutralization mechanism of three classes of human papillomavirus type 58 antibodies with disparate modes of binding." Journal of Virology 95.7 (2021): 10-1128. 2. under Open Access license cc BY 4.0. without modification.
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| Sub Cat | Clone | Type | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0724-FY12 | 5G9 | Mouse IgG2a | ||||||
| VS-0724-FY13 | 2H3 | Mouse IgG1 |
Specifications
- Immunogen
- Highly purified HPV58 VLPs.
- Host Species
- Mouse
- Specificity
- HPV58 L1
- Species Reactivity
- HPV58
- Applications
- Neutralization, Immunofluorescence, ELISA
- Related Disease
- Human Papillomavirus
Product Property
- Clonality
- Monoclonal Antibody
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Neutralization: 1 μg/mL
Immunofluorescence: 20 μg/mL
Target
- Alternative Names
- Human Papillomavirus type 58 L1; HPV58 L1
- UniProt ID
- P26535
- Long Name
- Human Papillomavirus type 58 L1
- Sequence Similarities
- Belongs to the papillomaviridae L1 protein family.
- Post Translation Modifications
- Disulfide bond
- Function
- The function of HPV58 L1 is to form the outer capsid of the human papillomavirus (HPV) virus. L1 is a major structural protein that self-assembles into pentameric capsomeres and further assembles to form the icosahedral capsid of the virus. The L1 protein plays a critical role in mediating viral entry, attachment to host cells, and immune evasion. Additionally, L1 is a major target for the development of HPV vaccines, as antibodies targeting L1 can prevent infection and viral replication.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-569LC | Anti-HPV58 L1 Recombinant Antibody (1A4) | ELISA | Mouse antibody |
| MRO-570LC | Anti-HPV58 L1 Recombinant Antibody (1C2) | ELISA | Mouse antibody |
| MRO-571LC | Anti-HPV58 L1 Recombinant Antibody (1D1) | ELISA | Mouse antibody |
| MRO-572LC | Anti-HPV58 L1 Recombinant Antibody (1D2) | ELISA | Mouse antibody |
| MRO-573LC | Anti-HPV58 L1 Recombinant Antibody (1G4) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-529 | Recombinant Mouse Anti-HPV58 L1 Antibody (A12A3) | ELISA, Neut, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-529 | Recombinant Mouse Anti-HPV58 L1 Antibody Fab Fragment (A12A3) | ELISA, Neut, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-529 | Recombinant Mouse Anti-HPV58 L1 Antibody scFv Fragment (A12A3) | ELISA, Neut, WB | Mouse scFv |
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