Mouse Anti-LPS core Recombinant Antibody (clone WN1-222-5) (CAT#: HPAB-1882-FY)

Figure 1 Shows the binding capacity of monoclonal antibody WNl 222-5 against different LPS molecules derived from eight different E. coli strains as determined by Western blotting.

The strains are described in the lane numbering represents: St = standard; 1 = E. coli 0111B4; 2 = E. coli 086; 3 = E. coli 018K"; 4 = E. coli 016; 5 = E. coli 015; 6 = E. coli 012; 7 = E. coli 06; and 8 = E. coli 04.

Figure 2 Reactivity of LPS, dLOS, and four lipid A derivatives with an mAb specific for the core OS region of LPS as determined by immunoblot analysis.

Lanes on the immunoblot: 1, LPS 2.5 mg; 2, dLOS 1 mg; 3, MPL 4 mg; 4, Kdo2-lipid A 4 mg; 5, GLA 4 mg; 6, detoxified lipid A from S. minnesota R595 4 mg; 7, dLOS 1 mg.

Han, J. E., Wui, S. R., Kim, K. S., Cho, Y. J., Cho, W. J., & Lee, N. G. (2014). Characterization of the structure and immunostimulatory activity of a vaccine adjuvant, de-O-acylated lipooligosaccharide. PLoS One, 9(1).

Figure 3 Reactivity of LPS, dLOS, and four lipid A derivatives with an mAb specific for the core OS region of LPS as determined by ELISA.

Han, J. E., Wui, S. R., Kim, K. S., Cho, Y. J., Cho, W. J., & Lee, N. G. (2014). Characterization of the structure and immunostimulatory activity of a vaccine adjuvant, de-O-acylated lipooligosaccharide. PLoS One, 9(1).

Figure 4 Interaction of WN1 222-5 with LPS and core oligosaccharides conjugated to BSA.

ELISA binding curves of the interaction of WN1 222-5 at different concentrations with LPS.

Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.

Figure 5 Binding of the E. coli F2513 (R4 core) oligosaccharide to WN1 222-5 in Hepes-buffered saline containing 150 mM NaCl.

The oligosaccharide concentration ranges were 0.5–10 M.

Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.

Figure 6 ITC microcalorimetry of WN1 222-5 binding to the E. coli F2513 (R4 core) oligosaccharide at 150 mM salt concentration.

N represents the number of binding sites; KA and K D are affinity and dissociation constants, respectively; and H is the enthalpy of binding.

Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.