Mouse Anti-LPS core Recombinant Antibody (clone WN1-222-5)
CAT#: HPAB-1882-FY
This product is a recombinant antibody that recognizes LPS core. The monoclonal antibody WN1-222-5 reacts with the epitope of LPS core.
Published Data
WB
Figure 1 Shows the binding capacity of monoclonal antibody WNl 222-5 against different LPS molecules derived from eight different E. coli strains as determined by Western blotting.
The strains are described in the lane numbering represents: St = standard; 1 = E. coli 0111B4; 2 = E. coli 086; 3 = E. coli 018K"; 4 = E. coli 016; 5 = E. coli 015; 6 = E. coli 012; 7 = E. coli 06; and 8 = E. coli 04.
WB
Figure 2 Reactivity of LPS, dLOS, and four lipid A derivatives with an mAb specific for the core OS region of LPS as determined by immunoblot analysis.
Lanes on the immunoblot: 1, LPS 2.5 mg; 2, dLOS 1 mg; 3, MPL 4 mg; 4, Kdo2-lipid A 4 mg; 5, GLA 4 mg; 6, detoxified lipid A from S. minnesota R595 4 mg; 7, dLOS 1 mg.
Han, J. E., Wui, S. R., Kim, K. S., Cho, Y. J., Cho, W. J., & Lee, N. G. (2014). Characterization of the structure and immunostimulatory activity of a vaccine adjuvant, de-O-acylated lipooligosaccharide. PLoS One, 9(1).
ELISA
Figure 3 Reactivity of LPS, dLOS, and four lipid A derivatives with an mAb specific for the core OS region of LPS as determined by ELISA.
Han, J. E., Wui, S. R., Kim, K. S., Cho, Y. J., Cho, W. J., & Lee, N. G. (2014). Characterization of the structure and immunostimulatory activity of a vaccine adjuvant, de-O-acylated lipooligosaccharide. PLoS One, 9(1).
ELISA
Figure 4 Interaction of WN1 222-5 with LPS and core oligosaccharides conjugated to BSA.
ELISA binding curves of the interaction of WN1 222-5 at different concentrations with LPS.
Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.
SPR
Figure 5 Binding of the E. coli F2513 (R4 core) oligosaccharide to WN1 222-5 in Hepes-buffered saline containing 150 mM NaCl.
The oligosaccharide concentration ranges were 0.5–10 M.
Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.
IA
Figure 6 ITC microcalorimetry of WN1 222-5 binding to the E. coli F2513 (R4 core) oligosaccharide at 150 mM salt concentration.
N represents the number of binding sites; KA and K D are affinity and dissociation constants, respectively; and H is the enthalpy of binding.
Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Gram-negative bacterial LPS core
- Species Reactivity
- Gram-negative bacterial
- Clone
- WN1-222-5
- Applications
- WB, ELISA, SPR, IA
- Related Disease
- Preliminary, transient and limited episode of bacteremia to a subsequent, fulminant and life-threatening conditon of endotoxemia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The LPS core antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance, Immunoassay.
Target
- Alternative Names
- Lipopolysaccharides core; LPS core
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone WN1-222-5"
- CAT
- Product Name
See other products for "LPS core"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1881-FY | Mouse Anti-LPS core Recombinant Antibody (clone WN1-58-9) | WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1881-FY-F(E) | Mouse Anti-LPS core Recombinant Antibody (clone WN1-58-9); scFv Fragment | WB | Mouse scFv |
| HPAB-1882-FY-F(E) | Mouse Anti-LPS core Recombinant Antibody (clone WN1-222-5); scFv Fragment | WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1881-FY-S(P) | Mouse Anti-LPS core Recombinant Antibody (clone WN1-58-9); Fab Fragment | WB | Mouse Fab |
| HPAB-1882-FY-S(P) | Mouse Anti-LPS core Recombinant Antibody (clone WN1-222-5); Fab Fragment | WB | Mouse Fab |
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