Camelid Anti-mCherry Recombinant Single Domain Antibody (FAMAB-0330-CN-VHH) (CAT#: FAMAB-0330-CN-VHH)

The antibody LaM-4 is an anti-mCherry VHH sdAb which binds to GFP with high affinity. The LaM-4 also binds to standard DsRed, which has approximately 80% sequence identity to mCherry.


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  • Published Data
  • Datasheet
  • MSDS
  • COA
IF

Figure 1 Efficacy of LaM sdAbs in immunofluorescence microscopy.

Figure 1 Efficacy of LaM sdAbs in immunofluorescence microscopy.

An S. cerevisiae strain with mCherry-tagged histone H2B was fixed and permeabilized, then directly stained with LaM-4 conjugated to Alexa Fluor® 488. Yeast were visualized in the green channels.

Fridy, P. C., Li, Y., Keegan, S., Thompson, M. K., Nudelman, I., Scheid, J. F., ... & Rout, M. P. (2014). A robust pipeline for rapid production of versatile sdAb repertoires. Nature methods, 11(12), 1253.


Specifications

  • Immunogen
  • Recombinant mCherry-His6
  • Host Species
  • Camelid
  • Derivation
  • Camelid
  • Type
  • Camelid VHH
  • Clone
  • FAMAB-0330-CN-VHH
  • Applications
  • IF

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Immunofluorescence. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.

Target

  • Alternative Names
  • mCherry

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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