Chimpanzee Anti-Norwalk Virus rVLPs Recombinant Antibody (clone B7) (CAT#: FAMAB-0264WJ)

This product is a Chimpanzee monoclonal antibody targets NV rVLPs. This antibody can be used in applications, such as ELISA, WB, IF, Neut.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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We require custom production
  • Published Data
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Binding specificities of anti-NV Fabs.

Figure 1 Binding specificities of anti-NV Fabs.

The binding profiles of Fabs B7, D8, E5, G4, and F11 at a concentration of 100 ng/ml on rVLPs from NV (GI.1), SW-2007 (GI.1), Hawaii (GII.1), and MD-145 (GII.4) that were attached to the solid phase at 1.5 μg/ml were measured by ELISA.

FC

Figure 2 Yeast cells expressing the c-myc-tagged monomeric VP1 fusion protein were analyzed by FACS analysis for VP1 expression and binding of VP1 by mouse anti-NV Mab NV4 and by chimpanzee anti-NV Fabs B7, D8, E5, and G4.

Figure 2 Yeast cells expressing the c-myc-tagged monomeric VP1 fusion protein were analyzed by FACS analysis for VP1 expression and binding of VP1 by mouse anti-NV Mab NV4 and by chimpanzee anti-NV Fabs B7, D8, E5, and G4.

Percentages indicate the numbers of cells gated by positive VP1 expression that were bound by antibody.

IF

Figure 3 Immunofluorescence staining results from Vero cells transfected with different DNA constructs.

Figure 3 Immunofluorescence staining results from Vero cells transfected with different DNA constructs.

Mutations of VP1 of Norwalk virus were introduced by using specific primers and a QuikChange site-directed mutagenesis kit according to the manufacturer's recommendations.

SPR

Figure 4 Representative sensorgram of Fab B7 analyzed by SPR.

Figure 4 Representative sensorgram of Fab B7 analyzed by SPR.

Showing raw data and curves fitted by using a 1:1 model.


Specifications

  • Immunogen
  • NV rVLPs
  • Host Species
  • Chimpanzee
  • Derivation
  • Phage Display Library
  • Type
  • Chimpanzee IgG
  • Specificity
  • NV rVLPs
  • Species Reactivity
  • Norwalk Virus
  • Clone
  • B7
  • Applications
  • ELISA, WB, IF, Neut
  • Related Disease
  • Norwalk Virus infection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Norwalk Virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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