PTM™ Rabbit Anti-Nos3 (pSer1177) Polyclonal Antibody (VS13-YC827) (CAT#: VS13-YC827)

• Immunogen
• Synthesised phosphopeptide of mouse eNOS at the site of Ser1177: TQ(pS)FS.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• The phosphorylated Nos3 (Ser1177) of human and mouse.

• Species Reactivity
• Human, Mouse, Rat (Predicted), Dog (Predicted), Pig (Predicted), Cow (Predicted), Rabbit (Predicted), Sheep (Predicted), Guinea pig (Predicted)

• Applications
• WB, ELISA, IHC-P, IHC-Fr, FC, IF

• Conjugate
• NONE

• Related Disease
• Aortic valve disease, Essential hypertension, Persistent fetal circulation syndrome, and Type 2 diabetes mellitus

• Clonality
• Polyclonal

• Purification
• Affinity Chromatography purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.

• Preservative
• 0.03% Proclin 300

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/2000
  ELISA: 1/5000-1/10000
  Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
  Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
  Flow cytometry (FC): 2 μg per 1x 10⁶ cells
  Immunofluorescence (IF): 1/100-1/500

• Alternative Names
• eNOS; Nos-3; ecNOS; 2310065A03Rik

• Gene ID
• 18127

• UniProt ID
• P70313

• Long Name
• nitric oxide synthase 3, endothelial cell

• Sequence Similarities
• Belongs to the NOS family.

• Cellular Localization
• Cell membrane; Cytoplasm; Cytoskeleton; Golgi apparatus

• Post Translation Modifications
• Phosphorylated

• Protein Refseq
• NP_000594.2; NP_001153581.1; NP_001153582.1

• Function
• Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832).
  NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody