Mouse Anti-NS1 Recombinant Antibody (HPAB-N0195-YC)
CAT#: HPAB-N0195-YC
The antibody thereof may specifically bind Dengue virus (DENV) serotype 4 and include: a heavy chain variable region that comprises at least one CDR amino acid sequence selected from the group consisting of: SGYNWH, YIH YS GGTN YNPS LKS, RTGTVPFAY, SYVMH, YLNPYNDDTKYNEKFKG, and GPPYALDY.
Specifications
- Immunogen
- SUMO-DENV4 rNS l fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- DENV4
- Species Reactivity
- DENV4
- Applications
- ELISA, WB
Applications
- Application Notes
- ELISA
NS1 capture ELISA was developed by utilizing two of the MAbs as a capture and detector antibody. In order to determine the optimal capture/detector pair for detection of DENV-4 NS1 antigen, each MAbs was tested either as a capture or a detector antibody. The optimal concentrations of capture and detector antibodies were determined by checkerboard titration ELISA. Microtiter plates were coated with MAb 8A6F2 (100 μl/well) diluted in bicarbonate buffer (pH 9.6) at concentration 10 μg/mL and incubated overnight at 4 °C. Next day, excess unbound capture MAb was removed and thereafter microtiter plates were blocked with 200 μl/well of 5% NFDM or 2% bovine serum albumin (BSA) (w/v). After 45 min incubation, microtiter plates washed 3X with PBST and thereafter 100 μl/ml of rNS1 antigen (0.5 μg/ml) and serum sample diluted 1:1 in PBST was added and incubated at 37 °C for 60 min. Following incubation and 3X washes, 100 μl/well of biotinylated MAb 6D4B10 diluted at 1:2000 in PBST were added and incubated for 1 h at 37 °C. After 3X washes, 100 μl/well peroxidase-conjugated Streptavidin was added and then microtiter plates were incubated at 37 °C for 30–60 min. After 5X washes, the reaction was visualized by adding 100 μl/well TMB liquid substrate and incubating at RT in the dark for 15 min. The reaction was then stopped with 100 μl/well TMB stop solution. The optical density (OD) was measured at 450 nm on ELISA ELx800 microplate reader.
Target
- Alternative Names
- Non-structural protein 1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "NS1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-712 | Recombinant Anti-NS1 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-712 | Recombinant Human Anti-NS1 Antibody | IF, FuncS | IgG |
| PABL-070 | Recombinant Human Anti-DENV NS1 Antibody (22NS1) | WB, ELISA, IF, FuncS | IgG |
| FAMAB-1386CQ | Human Anti-NS1 Recombinant Antibody (clone ZKA25) | ELISA | Human IgG1 |
| VS3-WK476 | Rabbit Anti-Ns1 Recombinant Antibody (clone JG37-82) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-712-F(E) | Recombinant Anti-NS1 Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-712-F(E) | Recombinant Human Anti-NS1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBL-070 | Recombinant Human Anti-DENV NS1 Antibody Fab Fragment (22NS1) | WB, ELISA, IF, FuncS | Fab |
| HPAB-N0195-YC-F(E) | Mouse Anti-NS1 Recombinant Antibody Fab Fragment (HPAB-N0195-YC-F(E)) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-712-S(P) | Recombinant Anti-NS1 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-712-S(P) | Recombinant Human Anti-NS1 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PSBL-070 | Recombinant Human Anti-DENV NS1 Antibody scFv Fragment (22NS1) | WB, ELISA, IF, FuncS | scFv |
| HPAB-N0195-YC-S(P) | Mouse Anti-NS1 Recombinant Antibody scFv Fragment (HPAB-N0195-YC-S(P)) | ELISA, WB | Mouse scFv |
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