Rabbit Anti-NTRK1 Recombinant Antibody (VS3-CJ367)
CAT#: VS3-CJ367
This product is a rabbit antibody that recognizes NTRK1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76570/1680_E8_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4606/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198400-NTRK1
❮
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NTRK1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
- Gene ID
- 4914
- UniProt ID
- P04629
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
- Cellular Localization
- Cell membrane, Endosome, Membrane
- Post Translation Modifications
- Ligand-mediated autophosphorylation (PubMed:2927393, PubMed:1281417, PubMed:15488758, PubMed:7510697, PubMed:8155326, PubMed:8325889, PubMed:27676246, PubMed:28177573).
Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1 (PubMed:15488758, PubMed:7510697, PubMed:8155326, PubMed:8325889).
N-glycosylated (PubMed:2927393).
Isoform TrkA-I and isoform TrkA-II are N-glycosylated
Ubiquitinated (PubMed:27445338).
Undergoes polyubiquitination upon activation; regulated by NGFR (PubMed:27445338).
Ubiquitination by NEDD4L leads to degradation (PubMed:27445338).
Ubiquitination regulates the internalization of the receptor (By similarity).
- Protein Refseq
- NP_001007793.1; NP_001012331.1; NP_002520.2
- Function
- Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1850821, PubMed:1849459, PubMed:1281417, PubMed:8325889, PubMed:15488758, PubMed:22649032, PubMed:17196528, PubMed:27445338).
Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity).
Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417).
Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
Isoform TrkA-III:
Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| HPAB-0728-CN-S(P) | Mouse Anti-NTRK1 Recombinant Antibody (clone CRB0022); scFv Fragment | WB, ELISA, FC | Mouse scFv |
| HPAB-0729-CN-S(P) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0088); scFv Fragment | WB, ELISA, FC | Human scFv |
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| HPAB-AP400-YC | Human Anti-NTRK1 Recombinant Antibody (clone CRB0036) | ELISA, FC, WB, Inhib | Human IgG |
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| HPAB-AP402-YC | Human Anti-NTRK1 Recombinant Antibody (clone CRB0072) | ELISA, FC, WB, Inhib | Human IgG |
| HPAB-AP403-YC | Human Anti-NTRK1 Recombinant Antibody (clone CRB0082) | ELISA, FC, WB, Inhib | Human IgG |
| HPAB-AP404-YC | Human Anti-NTRK1 Recombinant Antibody (clone CRB0084) | ELISA, FC, WB, Inhib | Human IgG |
| CAT | Product Name | Application | Type |
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| HPAB-AP400-YC-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0036); Fab Fragment | ELISA, FC, WB, Inhib | Human Fab |
| HPAB-AP401-YC-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0069); Fab Fragment | ELISA, FC, WB, Inhib | Human Fab |
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| HPAB-0659-YJ-F(E) | Mouse Anti-NTRK1 Recombinant Antibody (clone 5C3); Fab Fragment | Agonist, IHC, IF, Inhib | Mouse Fab |
| HPAB-0660-YJ-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone ch5C3); Fab Fragment | Agonist, IHC, IF, Inhib | Chimeric (mouse/human) Fab |
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