Porcine Anti-PDCoV N Recombinant Antibody (clone N53) (CAT#: VS-0323-CJ41)

Figure 1 Detection of the reactivity of selected scFvs with the PDCoV N protein by phage-ELISA with VS-0323-CJ41.

The plates were coated with purified PDCoV N protein and incubated with selected phages, followed by HRP-conjugated anti-M13 antibody for visualization. As negative control, 1% BSA was used.

Figure 2 Specificity of scFv N53 (VS-0323-CJ41) was determined by ELISA.

Plates that were coated with PDCoV, PEDV, TGEV and PSV were incubated with scFv N53, followed by HRP-conjugated anti-M13 antibody.

Figure 3 The reactivity of scFv N53 (VS-0323-CJ41) with the PDCoV N protein was analyzed by Western blot.

Lane 1, mock-infected ST cells; Lane 2, PDCoV-infected ST cells.

Figure 4 Reactivity of scFv N53 with PDCoV in ST cells was determined by IFA.

PDCoV-infected or mock-infected ST cells were incubated with scFv N53, followed by FITC-conjugated goat anti-human IgG (1:400 dilution). DAPI was used to visualize the cell nuclei.

Figure 5 Binding activity of scFv N53 (VS-0323-CJ41) to various truncated N epitope proteins was determined by dot blot.

The proteins were expressed and spotted on NC membrane, then incubated with scFv N53 and anti-His antibody, respectively. As a result, aa 82-96 of the PDCoV N protein was verified as the minimal epitope.