Porcine Anti-PDCoV N Recombinant Antibody (clone N53) (CAT#: VS-0323-CJ41)
This porcine anti-PDCoV N antibody (clone N53) is a product that recognizes Porcine deltacoronavirus (PDCoV) N protein.
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ELISA
Figure 1 Detection of the reactivity of selected scFvs with the PDCoV N protein by phage-ELISA with VS-0323-CJ41.
The plates were coated with purified PDCoV N protein and incubated with selected phages, followed by HRP-conjugated anti-M13 antibody for visualization. As negative control, 1% BSA was used.
ELISA
Figure 2 Specificity of scFv N53 (VS-0323-CJ41) was determined by ELISA.
Plates that were coated with PDCoV, PEDV, TGEV and PSV were incubated with scFv N53, followed by HRP-conjugated anti-M13 antibody.
WB
Figure 3 The reactivity of scFv N53 (VS-0323-CJ41) with the PDCoV N protein was analyzed by Western blot.
Lane 1, mock-infected ST cells; Lane 2, PDCoV-infected ST cells.
IF
Figure 4 Reactivity of scFv N53 with PDCoV in ST cells was determined by IFA.
PDCoV-infected or mock-infected ST cells were incubated with scFv N53, followed by FITC-conjugated goat anti-human IgG (1:400 dilution). DAPI was used to visualize the cell nuclei.
DB
Figure 5 Binding activity of scFv N53 (VS-0323-CJ41) to various truncated N epitope proteins was determined by dot blot.
The proteins were expressed and spotted on NC membrane, then incubated with scFv N53 and anti-His antibody, respectively. As a result, aa 82-96 of the PDCoV N protein was verified as the minimal epitope.
Specifications
- Immunogen
- PDCoV
- Host Species
- Porcine
- Type
- Porcine IgG
- Specificity
- Porcine deltacoronavirus (PDCoV) N protein
- Species Reactivity
- PDCoV
- Clone
- N53
- Applications
- Enzyme-linked Immunosorbent Assay, Immunofluorescence, Western Blot, Dot Blot
- Conjugate
- Unconjugated
- Related Disease
- Severe diarrhea and intestinal pathological damage in piglets
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 3-10 μg/mL
Western blot: 3-10 μg/mL
Dot blot: 3-10 μg/mL
Immunofluorescence: 20-40 μg/mL
Target
- Alternative Names
- Porcine deltacoronavirus N protein
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimeras
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY136 | Human Anti-PDCoV N (clone N53) scFv-Fc Chimera | ELISA, IF, Inhib | Human IgG1, scFv-Fc |
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