Porcine Anti-PEDV Recombinant Antibody (clone PZZ 35) (CAT#: VS-0323-CJ44)

Figure 1 The antibodies (scFv version) were further analyzed by phage ELISA.

An scFv that showed no affinity to PEDV antigen was used as a negative control. These scFvs were designated PZZ 21 (VS-0323-CJ42), PZZ 24 (VS-0323-CJ43), and PZZ 35 (VS-0323-CJ44), respectively.

Figure 2 VPZZ 35 (VS-0323-CJ44) bound to PEDV-infected cells.

PEDV-infected Vero E6 cells were incubated with PZZ 35 (VS-0323-CJ44) at 24 h post-infection and then stained with the FITC conjugated anti-His monoclonal antibody.

Figure 3 Viability of the cells after treatment with different concentrations of purified scFv by the MTT assay.

Vero E6 cells or porcine IPEC-J2 cells were incubated with 2-fold dilution of purified scFv (3.125-200 μg/mL), and the cell viability was calculated using the MTT assay kit.

Figure 4 Neutralizing activity of scFv against PEDV

An 80% reduction in the number of plaques was used as a cutoff to determine neutralizing antibody titers.

Figure 5 VS-0323-CJ44 binds to the spike (S) protein in PEDV-infected cells.

Figure 6 Piglets orally administered scFvs survived after PEDV infection.