Recombinant Mouse Anti-pNB and pNP ester Catalytic Antibody (D2.4) (CAT#: FAMAB-0312YC)
Provided is a catalytic antibody capable of hydrolysing p-nitrobenzyl (pNB) and p-nitrophenyl (pNP) esters. The catalytic antibody exhibited high reactivity and substrate specificity.
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Custom Production
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ELISA
Figure 1 Relative affinities of antibody D2.4 to the Nbzl-phosphonate and Np-phosphonate (1b, lp) and substrates (2b, 2p,) were determined by competitive inhibition immunoassay (CIEIA).
D2.4 (0.5 nM) was previously incubated with different concentrations of these substances at pH 6.0.
Tawfik, D. S., Lindner, A. B., Chap, R., Eshhar, Z., & Green, B. S. (1997). Efficient and Selective P‐nitrophenyl‐ester‐hydrolyzing Antibodies Elicited by a P‐nitrobenzyl Phosphonate Hapten. European journal of biochemistry, 244(2), 619-626.
Specifications
- Immunogen
- A nitrobenzyl phosphonate conjugated to KLH
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- pNB and pNP ester
- Clone
- D2.4
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
- Alternative Names
- pNB and pNP ester; p-nitrobenzyl and p-nitrophenyl ester
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0311YC | Recombinant Mouse Anti-pNB and pNP ester Catalytic Antibody (D2.3) | ELISA | Mouse IgG2a, κ |
| FAMAB-0313YC | Recombinant Mouse Anti-pNB and pNP ester Catalytic Antibody (D2.5) | ELISA | Mouse IgG2a, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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