Mouse Anti-Polycation Recombinant Antibody (clone ALl-2) (CAT#: FAMAB-0271CQ)

Figure 1 Binding of the mAb to cationic antigens.

After its initial purification, mAb ALl-2 was dissolved in PBS containing 3 M NaCl and re-purified on to a recombinant protein G-agarose column. After washing the column with PBS containing 3 M NaCI, the mAb was eluted in 0.1 M glycine-HCl pH 2.8 and dialysed against standard PBS (0.15 M NaCl). This procedure rids mAb from potential contaminants present in the culture medium such as DNA. mAb (before and after 3 M NaCl treatment) was then tested against several cationic antigens. mAb was used at 0.2 μg/ml ALI-2 in the assay.

Monestier, M., Stemmer, C., Ong, G. L., & Muller, S. (1997). Induction of anti-polycation antibodies in H-2s mice by immunization with nuclear antigens. Molecular immunology, 34(1), 39-51.

Figure 2 Inhibition of mAb binding to poly-L-lysine by pre-incubation with polycations in solution.

In this assay, the mAb at constant concentration (0.25 μg/ml for ALl-2) was pre-incubated in solution with varying concentrations of poly-L-lysine, poly-L-arginine or spermidine-BSA. The mixtures were then added onto poly-L-lysine-coated plates and the remainder of the ELISA was conducted. The results are expressed as percent inhibition of the binding measured in the absence of competitor.

Monestier, M., Stemmer, C., Ong, G. L., & Muller, S. (1997). Induction of anti-polycation antibodies in H-2s mice by immunization with nuclear antigens. Molecular immunology, 34(1), 39-51.

Figure 1 Mouse Anti-Polycation Recombinant Antibody (FAMAB-0271CQ) in SDS-PAGE

Lane 1: Non-reduced mAb
Lane 2: Reduced mAb