Rabbit Anti-RPA1 Recombinant Antibody (clone R01-3F4) (CAT#: VS3-FY2081)

• Immunogen
• Synthetic peptide of human RPA70

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human RPA1

• Species Reactivity
• Human

• Clone
• R01-3F4

• Applications
• Western Blot, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 68 kDa, while the observed molecular weight stands at 68 kDa.

• Related Disease
• Pulmonary Fibrosis And/Or Bone Marrow Failure Syndrome, Telomere-Related, 6 and Ataxia-Telangiectasia

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• Replication Protein A1; Single-Stranded DNA-Binding Protein; Replication Factor A Protein 1; Replication Protein A1, 70kDa; RF-A Protein 1; RP-A P70; REPA1; RPA70

• Gene ID
• 6117

• UniProt ID
• P27694

• Sequence Similarities
• Belongs to the replication factor A protein 1 family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR (PubMed:24332808)., Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination (PubMed:26474068)., Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6.

• Protein Refseq
• NP_002936.1

• Function
• As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism.
  Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
  In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.
  It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
  Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair.
  Also plays a role in base excision repair (BER) probably through interaction with UNG.
  Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. Plays a role in telomere maintenance.
  As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody