Mouse Anti-RUBCN Recombinant Antibody (clone 4F5B5)
CAT#: VS7-0425-WR802
This product is a mouse IgG1 antibody that binds to human RUBCN. The antibody 4F5B5 recognizes specific amino acid domain of human NR2C2 (AA: 62-356). It can be used for WB and ELISA assays.
Specifications
- Immunogen
- Recombinant human NR2C2 (AA: 62-356) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RUBCN
- Species Reactivity
- Human
- Clone
- 4F5B5
- Molecule Class
- Negative Regulator
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Spinocerebellar ataxia, autosomal recessive, 15 (SCAR15)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SCAR15; RUBICON; KIAA0226
- Gene ID
- 9711
- UniProt ID
- Q92622
- Long Name
- Rubicon Autophagy Regulator
- Cellular Localization
- Endosome; Lysosome
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001139114.1
- Function
- Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation.; Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines.; Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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