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Rabbit Anti-SDC3 Recombinant Antibody (clone R04-6B2)

Rabbit Anti-SDC3 Recombinant Antibody (clone R04-6B2) (CAT#: VS3-FY1888)

Name: Rabbit Anti-SDC3 Recombinant Antibody (clone R04-6B2)
SKU: VS3-FY1888
Rating: 5 (1 reviews)

The R04-6B2 recombinant monoclonal antibody targets for human SDC3. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. This syndecan 3 antibody has been teted positive by western blot analysis of syndecan 3 in K562, C6, 3T3 lysates.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Anti-SDC3 Antibody (VS3-FY1888) in WB.

Figure 1 Anti-SDC3 Antibody (VS3-FY1888) in WB.

The Syndecan 3 expression in K562, C6, 3T3 lysates were detected using the anti-Syndecan 3 by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48085/798_H1_3_selected.jpg

Normal Tissue

Figure 2 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/ihc_selected.jpg

Figure 3 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25786/58734_B_6_5_selected.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/40654_A_9_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/40654_A_6_6.jpg

Figure 6 Placenta
(Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/40654_A_1_7.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (SK-MEL-30, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162512-SDC3

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human Syndecan 3

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat SDC3

Species Reactivity
Human, Mouse, Rat

Clone
R04-6B2

Applications
Western Blot, Immunoprecipitation

MW
The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 60 kDa.

Related Disease
Body Mass Index Quantitative Trait Locus 11 and Alcohol-Induced Mental Disorder

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20

Target

Alternative Names
Syndecan 3; Syndecan Proteoglycan 3; SYND3; Syndecan 3 (N-Syndecan); Syndecan Neural Type; N-Syndecan; Syndecan-3; KIAA0468; SDCN

Gene ID
9672

UniProt ID
O75056

Sequence Similarities
Belongs to the syndecan proteoglycan family.

Cellular Localization
Cell membrane, Membrane

Post Translation Modifications
O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules. (O75056-SDC3_HUMAN)
Glycosylation at Ser80, Ser82, Ser84, Ser91, Ser108, Thr109, Thr110, Ser160, Thr161, Thr162, Thr169, Ser170, Thr171, Ser250, Thr251, Thr253, Thr264, Thr265, Ser314, Ser344, Thr349, and Ser367 (NX_O75056 [NX_O75056-1])
Modification sites at PhosphoSitePlus (O75056)
Glycosylation from GlyGen (O75056) 30 sites, 4 O-linked glycans (15 sites)

Protein Refseq
XP_011540765.1; XP_011540766.1; XP_011540768.1; XP_054195715.1; XP_054195716.1

Function
May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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