Rabbit Anti-SDC3 Recombinant Antibody (clone R04-6B2)
CAT#: VS3-FY1888
The R04-6B2 recombinant monoclonal antibody targets for human SDC3. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. This syndecan 3 antibody has been teted positive by western blot analysis of syndecan 3 in K562, C6, 3T3 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48085/798_H1_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25786/58734_B_6_5_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/40654_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/40654_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17087/40654_A_1_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (SK-MEL-30, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162512-SDC3
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WB
Figure 1 Anti-SDC3 Antibody (VS3-FY1888) in WB.
The Syndecan 3 expression in K562, C6, 3T3 lysates were detected using the anti-Syndecan 3 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Syndecan 3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SDC3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-6B2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 60 kDa.
- Related Disease
- Body Mass Index Quantitative Trait Locus 11 and Alcohol-Induced Mental Disorder
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Syndecan 3; Syndecan Proteoglycan 3; SYND3; Syndecan 3 (N-Syndecan); Syndecan Neural Type; N-Syndecan; Syndecan-3; KIAA0468; SDCN
- Gene ID
- 9672
- UniProt ID
- O75056
- Sequence Similarities
- Belongs to the syndecan proteoglycan family.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules. (O75056-SDC3_HUMAN)
Glycosylation at Ser80, Ser82, Ser84, Ser91, Ser108, Thr109, Thr110, Ser160, Thr161, Thr162, Thr169, Ser170, Thr171, Ser250, Thr251, Thr253, Thr264, Thr265, Ser314, Ser344, Thr349, and Ser367 (NX_O75056 [NX_O75056-1])
Modification sites at PhosphoSitePlus (O75056)
Glycosylation from GlyGen (O75056) 30 sites, 4 O-linked glycans (15 sites)
- Protein Refseq
- XP_011540765.1; XP_011540766.1; XP_011540768.1; XP_054195715.1; XP_054195716.1
- Function
- May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SDC3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3839z | Mouse Anti-SDC3 Recombinant Antibody (clone 42B12) | WB, IP, IF, ELISA | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3190 | Hi-Affi™ Recombinant Rabbit Anti-SDC3 Monoclonal Antibody (DS3190AB) | WB, IP | IgG |
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