Mouse Anti-SH3GL1 Recombinant Antibody (clone 2A9H4)
CAT#: VS7-0425-WR850
This product is a mouse IgG1 antibody that binds to human SH3GL1. The antibody 2A9H4 recognizes specific amino acid domain of human SH3GL1 (AA: 12-119). It can be used for FC assay.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_B_5_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_B_8_5.jpg
Normal Tissue
Figure 3 Oral mucosa
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_A_9_1.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21485/49412_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141985-SH3GL1
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Specifications
- Immunogen
- Recombinant human SH3GL1 (AA: 12-119) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SH3GL1
- Species Reactivity
- Human
- Clone
- 2A9H4
- Molecule Class
- Endophilin A2
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SH3GL1; Extra Eleven-Nineteen Leukemia Fusion Gene Protein; SH3P8; SH3-Containing Grb-2-Like 1 Protein; SH3 Domain-Containing GRB2-Like Protein 1; EEN Fusion Partner Of MLL; Endophilin-2; Fusion Partner Of MLL; EEN; SH3 Domain GRB2-Like 1; CNSA1; Extra 11
- Gene ID
- 6455
- UniProt ID
- Q99961
- Long Name
- SH3 Domain Containing GRB2 Like 1, Endophilin A2
- Cellular Localization
- Cell junction; Cell projection; Cytoplasm; Endosome; Membrane
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001186872.1
- Function
- Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Reconstitution Buffer
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2888z | Mouse Anti-SH3GL1 Recombinant Antibody (clone 22A11) | WB, FC, IF, IHC | Mouse IgG1 |
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| VS3-WK169 | Mouse Anti-SH3GL1 Recombinant Antibody (clone H4-A9) | WB, ELISA | Mouse IgG1 |
| VS7-0425-WR851 | Mouse Anti-SH3GL1 Recombinant Antibody (clone 4B4C2) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0534MZ | Chicken Anti-SH3GL1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6552 | Anti-SH3GL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6553 | Anti-Mouse SH3GL1 Immunohistochemistry Kit | IHC |
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