Rat Anti-SMTSP2 Recombinant Antibody (clone T2)
CAT#: VS-0623-WK4
The antibody clone T2 was produced by immunizing rat with recombinant protein corresponding to S. mansoni immunogenic protein SmTsp2. Western blotting with a pool of T2 recognizes an apical membrane protein of similar but not identical molecular weight to Sm23 that is expected to be native SmTsp2. It can be used for detection of S. mansoni SmTsp2 in ELISA and WB assays.
Published Data
WB
Figure 1 Western blot analysis of the rat scFv recognition of Sm23/MBP and SmTsp2/MBP.
MBP-fusion protein were separated by a 12% PAGE-SDS, incubated overnight with scFv and detected with anti-E-tag mAb. Lane 1 contains purified Sm23/MBP, Lane 2 contains purified SmTsp2/MBP.
WB
Figure 2 Western blot analysis of rat scFv recognition of S. mansoni tegumental antigens.
Lane 1 used a control rat scFv. Lane 2 used a pool of scFvs S1 and S3. Lane 3 used a pool of scFvs T1 and T2. The tegumental extract was resolved by 12% PAGE-SDS. Bound scFv was detected with anti-E-tag mAb.
ELISA
Figure 3 ELISA analysis of scFv (30 nM) recognition of Sm23/MBP (grey bars), SmTsp2/MBP (striped bars) and S. mansoni tegumental extract (black bars).
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Specifications
- Immunogen
- Recombinant protein corresponding to S. mansoni immunogenic protein SmTsp2
- Host Species
- Rat
- Type
- Rat IgG
- Specificity
- S. mansoni SmTsp2
- Species Reactivity
- S. mansoni
- Clone
- T2
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The scFv (30 nM) antibody product was tested in ELISA recognition of Sm23/MBP, SmTsp2/MBP and S. mansoni tegumental extract.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0623-WK3 | Rat Anti-SMTSP2 Recombinant Antibody (clone T1) | ELISA, WB | Rat IgG |
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