This is a chimeric mouse-human monoclonal antibody (cMAb) against BP and/or BM. The cMAb BP7 2C6 (cMAb CK2) recognizes surface-capsule antigens with molecular sizes of 38 to 52 kDa, and 200 kDa in BM.
Figure 1 Binding property of 3 cMAbs produced by CHO-DG44 cells without DHFR amplification.
For ELISA, the pIRES-H(L)-DHFR vectors containing chimeric H-chain (H1, H2, and H3) or L-chain (L1, L2, and L3) were cotransfected into CHO-DG44 cells using a LF-2000 reagent and the master seed cells were incubated for 3 days to get sufficient amounts of cMAbs before the DHFR-amplification process.
Figure 2 Western immunoblot analysis of BP, BM, and BT with 3 cMAbs.
For Western blot analysis, 3 cMAb culture supernatants (cMAbs CK1, CK2, and CK3) were used at 1:5 dilutions.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0243-CN-S(P) | Recombinant Mouse Anti-Surface-capsule antigens Antibody scFv Fragment (CK2) | WB, ELISA | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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