GeneSpark™ Human CaMK2D Intron 3 Primers (VS-0725-FY80)

CAT#: VS-0725-FY80

The Human CaMK2D Intron 3 Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target intron 3 of the human CaMK2D (Calcium/Calmodulin Dependent Protein Kinase II Delta) gene. CaMK2D is a serine/threonine protein kinase involved in calcium signaling, learning, and memory, with diverse splice variants. Investigating intronic regions can provide insights into chromatin states or RNA polymerase progression within the gene body. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.

Specifications

  • Application
  • ChIP
  • Application Notes
  • Once bound to their target DNA, primers serve as the starting points for DNA amplification, typically through quantitative Polymerase Chain Reaction (qPCR) or next-generation sequencing (ChIP-seq).
  • Workflow
  • 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
    2. Add 2 μl ChIP DNA sample per well/tube.
    3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
    4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
    5. Analyze results with real-time PCR software.
  • Size
  • 0.5 mL
  • Components
  • These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
  • Biological Samples
  • Human
  • Storage
  • Store at -20°C.

Target

  • Full Name
  • Calcium/Calmodulin Dependent Protein Kinase II Delta
  • Introduction
  • CAMK2D (Calcium/Calmodulin Dependent Protein Kinase II Delta) is a Protein Coding gene. Diseases associated with CAMK2D include Dilated Cardiomyopathy. Among its related pathways are RET signaling and Salivary secretion. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and protein kinase activity. An important paralog of this gene is CAMK2B.
  • Alternative Names
  • Calcium/Calmodulin Dependent Protein Kinase II Delta; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Delta; CaM Kinase II Subunit Delta; CaMK-II Subunit Delta; EC 2.7.11.17; CAMKD; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Delta
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