GeneSpark™ Human DMD Intron 2 Primers (VS-0725-FY68)
CAT#: VS-0725-FY68
The Human DMD Intron 2 Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target intron 2 of the human DMD (Dystrophin) gene. Dystrophin is a crucial protein for muscle function, and mutations in this gene lead to Duchenne and Becker muscular dystrophies. Targeting an intronic region allows for the study of chromatin modifications or transcription factor binding within the gene body. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers are crucial in Chromatin Immunoprecipitation (ChIP) assays. They function as precise probes designed to identify and quantify specific DNA sequences that have been enriched during the immunoprecipitation process. This targeted detection is essential for uncovering and mapping protein-DNA interactions, providing critical insights into gene regulation and chromatin structure. Essentially, primers enable researchers to pinpoint exactly where proteins bind to DNA.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Dystrophin
- Introduction
- This gene spans a genomic range of greater than 2 Mb and encodes a large protein containing an N-terminal actin-binding domain and multiple spectrin repeats. The encoded protein forms a component of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. Deletions, duplications, and point mutations at this gene locus may cause Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), or cardiomyopathy. Alternative promoter usage and alternative splicing result in numerous distinct transcript variants and protein isoforms for this gene.
- Alternative Names
- Dystrophin; Dystrophin (Muscular Dystrophy, Duchenne And Becker Types), Includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272; Muscular Dystrophy, Duchenne And Becker Types; Mental Retardation, X-Linked 85; DXS142; DXS164; DXS206; DXS230; DXS239
- Gene ID
- 1756
- UniProt ID
- P11532
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