GeneSpark™ Human FOXN3 Intron 3 Primers (VS-0725-FY46)
CAT#: VS-0725-FY46
The Human FOXN3 Intron 3 Primers are designed for amplifying DNA isolated through chromatin immunoprecipitation (ChIP). This product includes optimized forward and reverse PCR primers specific to intron 3 of the human FOXN3 gene. FOXN3 is a forkhead box transcription factor involved in various cellular processes, including cell cycle regulation and differentiation. Targeting an intron region can provide insights into chromatin accessibility or RNA polymerase progression within the gene body. These primers are optimized for SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers in Chromatin Immunoprecipitation (ChIP) assays serve as precise tools for pinpointing and measuring specific DNA sequences that have been enriched through immunoprecipitation. This process is crucial for uncovering protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Forkhead Box N3
- Introduction
- This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms.
- Alternative Names
- Forkhead Box N3; Checkpoint Suppressor 1; C14orf116; CHES1; Chromosome 14 Open Reading Frame 116; Forkhead Box Protein N3; PRO1635
- Gene ID
- 1112
- UniProt ID
- O00409
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