GeneSpark™ Human HNRNPA0 Promoter Primers (VS-0725-FY45)
CAT#: VS-0725-FY45
The Human HNRNPA0 Promoter Primers offer a precise tool for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product provides a pre-mixed set of forward and reverse PCR primers specifically targeting the promoter region of the human HNRNPA0 (Heterogeneous Nuclear Ribonucleoprotein A0) gene. HNRNPA0 is involved in RNA processing and transport, and its promoter activity is generally constitutive. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been rigorously tested with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are valuable for studying basal gene regulation and active chromatin marks.
Specifications
- Application
- ChIP
- Application Notes
- Primers in Chromatin Immunoprecipitation (ChIP) assays serve as precise tools for pinpointing and measuring specific DNA sequences that have been enriched through immunoprecipitation. This process is crucial for uncovering protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Heterogeneous Nuclear Ribonucleoprotein A0
- Introduction
- This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
- Alternative Names
- Heterogeneous Nuclear Ribonucleoprotein A0; HnRNP A0; HNRPA0; HnRNA Binding Protein
- Gene ID
- 10949
- UniProt ID
- Q13151
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