GeneSpark™ Human IKZF3 Promoter Primers (VS-0725-FY41)
CAT#: VS-0725-FY41
The Human IKZF3 Promoter Primers offer a highly specific solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human IKZF3 (IKAROS family zinc finger 3) gene, also known as Aiolos. IKZF3 is a transcription factor important in lymphocyte development and function, playing roles in B cell differentiation and antibody production. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been rigorously tested with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are valuable for studying gene regulation in immunology.
Specifications
- Application
- ChIP
- Application Notes
- The primary role of primers in chromatin immunoprecipitation (ChIP) assays is to act as precise probes for identifying and quantifying particular DNA sequences that have been enriched through immunoprecipitation, thereby uncovering protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- IKAROS Family Zinc Finger 3
- Introduction
- This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants.
- Alternative Names
- IKAROS Family Zinc Finger 3
- Gene ID
- 22806
- UniProt ID
- Q9UKT9
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