GeneSpark™ Human LENG8 5' UTR Primers (VS-0725-FY47)
CAT#: VS-0725-FY47
The Human LENG8 5' UTR Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the 5' Untranslated Region (UTR) of the human LENG8 gene. The 5' UTR can be involved in translational control and often reflects transcriptional activity near the gene start. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are useful for investigating epigenetic marks near the transcriptional start site.
Specifications
- Application
- ChIP
- Application Notes
- Primers in Chromatin Immunoprecipitation (ChIP) assays serve as precise tools for pinpointing and measuring specific DNA sequences that have been enriched through immunoprecipitation. This process is crucial for uncovering protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
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