GeneSpark™ Human Negative Control Primer Set 1 (VS-0725-FY104)
CAT#: VS-0725-FY104
The Human Negative Control Primer Set 1 is designed to serve as a crucial negative control in chromatin immunoprecipitation (ChIP) assays with human samples. This set features a meticulously validated mixture of forward and reverse primers that target a region of the genome not expected to be enriched by most common protein-DNA interactions. It's essential for assessing background noise and confirming the specificity of your ChIP antibodies, ensuring that any detected signal is truly from specific enrichment rather than non-specific binding or contamination.
Specifications
- Application
- ChIP
- Application Notes
- Human Control qPCR Primer Sets are engineered for use as either positive or negative controls in chromatin immunoprecipitation (ChIP) assays involving human samples. Each set provides a validated mixture of forward and reverse primers, suitable for both qPCR and endpoint PCR analysis of ChIP samples across various human cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Human
- Storage
- Store at -20°C.
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