GeneSpark™ Human Positive Control Primer Set GAPDH-1 (VS-0725-FY110)
CAT#: VS-0725-FY110
The Human Positive Control Primer Set GAPDH-1 provides an essential positive control for human ChIP experiments. Targeting a well-characterized region of the GAPDH gene, this primer set consists of a meticulously validated blend of forward and reverse primers. It guarantees robust and consistent amplification in both qPCR and endpoint PCR applications, allowing researchers to confidently assess the success of their immunoprecipitation steps and the overall integrity of their ChIP samples.
Specifications
- Application
- ChIP
- Application Notes
- Human Control qPCR Primer Sets are engineered for use as either positive or negative controls in chromatin immunoprecipitation (ChIP) assays involving human samples. Each set provides a validated mixture of forward and reverse primers, suitable for both qPCR and endpoint PCR analysis of ChIP samples across various human cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Human
- Storage
- Store at -20°C.
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