GeneSpark™ Human ZNF19 Intron 3 Primers (VS-0725-FY48)
CAT#: VS-0725-FY48
The Human ZNF19 Intron 3 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting intron 3 of the human ZNF19 (Zinc Finger Protein 19) gene. ZNF19 is a transcription factor, and its intronic regions can provide insights into chromatin organization or transcriptional elongation. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been thoroughly validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They facilitate the study of chromatin dynamics within the gene body.
Specifications
- Application
- ChIP
- Application Notes
- Primers in Chromatin Immunoprecipitation (ChIP) assays serve as precise tools for pinpointing and measuring specific DNA sequences that have been enriched through immunoprecipitation. This process is crucial for uncovering protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Zinc Finger Protein 19
- Introduction
- The protein encoded by this gene contains a zinc finger, a nucleic acid-binding domain present in many transcription factors. This gene is located in a region next to ZNF23, a gene also encoding a zinc finger protein, on chromosome 16.
- Alternative Names
- Zinc Finger Protein 19, Zinc Finger Protein 19 (KOX 12), Zinc Finger Protein KOX12, KOX12
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Datasheet
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