GeneSpark™ Human ZNF19 Intron 3 Primers (VS-0725-FY48)

CAT#: VS-0725-FY48

The Human ZNF19 Intron 3 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting intron 3 of the human ZNF19 (Zinc Finger Protein 19) gene. ZNF19 is a transcription factor, and its intronic regions can provide insights into chromatin organization or transcriptional elongation. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been thoroughly validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They facilitate the study of chromatin dynamics within the gene body.

Specifications

  • Application
  • ChIP
  • Application Notes
  • Primers in Chromatin Immunoprecipitation (ChIP) assays serve as precise tools for pinpointing and measuring specific DNA sequences that have been enriched through immunoprecipitation. This process is crucial for uncovering protein-DNA interactions.
  • Workflow
  • 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
    2. Add 2 μl ChIP DNA sample per well/tube.
    3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
    4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
    5. Analyze results with real-time PCR software.
  • Size
  • 0.5 mL
  • Components
  • These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
  • Biological Samples
  • Human
  • Storage
  • Store at -20°C.

Target

  • Full Name
  • Zinc Finger Protein 19
  • Introduction
  • The protein encoded by this gene contains a zinc finger, a nucleic acid-binding domain present in many transcription factors. This gene is located in a region next to ZNF23, a gene also encoding a zinc finger protein, on chromosome 16.
  • Alternative Names
  • Zinc Finger Protein 19, Zinc Finger Protein 19 (KOX 12), Zinc Finger Protein KOX12, KOX12
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