GeneSpark™ Mouse HoxD10 Exon 1 Primers (VS-0725-FY59)
CAT#: VS-0725-FY59
The Mouse HoxD10 Exon 1 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting exon 1 of the mouse HoxD10 gene. HoxD10 is a homeobox gene within the HoxD cluster, playing a critical role in limb development and axial patterning. Investigating this exonic region can provide insights into transcription factor binding or active chromatin marks within a transcribed region. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- The primary role of primers in chromatin immunoprecipitation (ChIP) assays is to act as accurate detectors. They enable the identification and measurement of specific DNA sequences that have been concentrated through immunoprecipitation, thereby illuminating protein-DNA binding events.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- Homeobox D10
- Introduction
- This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as "rocker-bottom foot" deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease.
- Alternative Names
- Homeobox D10; Homeobox Protein Hox-4D; Homeobox Protein Hox-4E; Homeo Box D10; HOX4D; HOX4E
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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